Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	533,695	T→A	I5F (ATC→TTC)	ybhS ←	predicted transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	533,695	0	T	A	100.0%	43.9 / NA	15	I5F (ATC→TTC)	ybhS	predicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/0);  total (15/0)

GATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCGGGTTA  >  minE/533642‑533702                                                      |        gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:165420/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:178953/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:2022207/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:2037260/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:2043510/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:2440341/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:2746592/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:2820575/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:291443/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:470628/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:601047/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:669656/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:726148/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:764249/1‑61 (MQ=255) gATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGAACGGGTTa  >  1:780502/1‑61 (MQ=255)                                                      |        GATCTGCCGCGTCTCTTTAACGCACAGCGCCCGTACGCGACGCCAGGACAGGATCGGGTTA  >  minE/533642‑533702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑