Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	710,816	G→A	A200V (GCG→GTG)	solA ←	N‑methyltryptophan oxidase, FAD‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	710,816	0	G	A	100.0%	67.2 / NA	22	A200V (GCG→GTG)	solA	N‑methyltryptophan oxidase, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/11);  total (11/11)

CGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATA  >  minE/710769‑710866                                                |                                                   cGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCACGCAGACAATCGCt                                      >  1:1031751/1‑62 (MQ=255) cGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCACGCAGACAATCGCt                                      >  1:13265/1‑62 (MQ=255) cGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCACGCAGACAATCGCt                                      >  1:1586428/1‑62 (MQ=255)          cAGGCAGCTCCGGGAGCAGGTCTTTTATCCATGTTCCCACGCAGACAATCGCTTTTTTCg                               <  1:934713/60‑1 (MQ=255)          cAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTACCCACGCAGACAATCGCTTTTTTCg                               <  1:1009138/60‑1 (MQ=255)                              tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:1012674/62‑1 (MQ=255)                              tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:979760/62‑1 (MQ=255)                              tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:560126/62‑1 (MQ=255)                              tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:50449/62‑1 (MQ=255)                              tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:445384/62‑1 (MQ=255)                              tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:360079/62‑1 (MQ=255)                              tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:3097078/62‑1 (MQ=255)                              tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:1794359/62‑1 (MQ=255)                              tCTTTTACCCATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGt         <  1:1118488/62‑1 (MQ=255)                                      ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:3185149/1‑61 (MQ=255)                                      ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:3215204/1‑61 (MQ=255)                                      ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:2867151/1‑61 (MQ=255)                                      ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:2072631/1‑61 (MQ=255)                                      ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:1186542/1‑61 (MQ=255)                                      ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:784778/1‑61 (MQ=255)                                      ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:1140530/1‑61 (MQ=255)                                      ccATGTTCCCACGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATa  >  1:99001/1‑61 (MQ=255)                                                |                                                   CGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATA  >  minE/710769‑710866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑