Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	724,065	T→C	T103T (ACT→ACC)	yceQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	724,065	0	T	C	100.0%	216.4 / NA	57	T103T (ACT→ACC)	yceQ	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/55);  total (2/55)

CCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGC  >  minE/724006‑724110                                                            |                                              ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:2643658/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:3017122/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:2022869/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:2921975/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:2394946/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:2403156/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:602436/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:2474302/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:2849948/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:1401782/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:1393274/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:1367803/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:807478/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:1342713/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:814146/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:2761132/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAcctc                                             >  1:1286359/1‑62 (MQ=255) ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCATACTCACAGTTTTTTCAcctc                                             >  1:3044327/1‑62 (MQ=255)                              ttATCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCaa                <  1:1736352/62‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:278017/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2961368/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2986147/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:30029/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:3004447/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2737228/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:437617/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:3044464/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:3072946/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:3125683/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:3190832/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1054271/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:445624/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:468761/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:518140/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:52028/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:52411/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:641328/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:701999/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:7071/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:838255/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:85861/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:912602/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1019936/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1333123/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1353725/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1434809/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1454571/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1497649/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1517924/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1519156/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1547243/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1584935/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1637506/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1704774/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1897588/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2580710/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:197234/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2093692/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:252762/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2110033/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2113684/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:218785/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2190197/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2205962/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2041140/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:235562/61‑1 (MQ=255)                                 tCACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2214940/61‑1 (MQ=255)                                  cACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2214468/60‑1 (MQ=255)                                  cACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:405376/60‑1 (MQ=255)                                  cACCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGATTAATAACCATCAATa              <  1:1323994/60‑1 (MQ=255)                                   aCCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2977733/59‑1 (MQ=255)                                   aCCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:2515956/59‑1 (MQ=255)                                   aCCCGCTTACTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATa              <  1:1244812/59‑1 (MQ=255)                                            cTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGc  >  1:3274501/1‑62 (MQ=255)                                            cTCACAGTTTTTTCACCTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGc  >  1:2544130/1‑62 (MQ=255)                                                            |                                              CCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGC  >  minE/724006‑724110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑