Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	799,902	A→G	intergenic (‑312/+8)	treA ← / ← dhaH	periplasmic trehalase/fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	799,902	0	A	G	100.0%	60.4 / NA	17	intergenic (‑312/+8)	treA/dhaH	periplasmic trehalase/fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/0);  total (17/0)

CATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAACAGTGGCTTAAC  >  minE/799853‑799914                                                  |             cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:2691616/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:559485/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:548207/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:547967/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:463601/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:37026/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:363553/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:3132498/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:3078451/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:1318492/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:2550675/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:2474712/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:2326303/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:2154713/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:1874345/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:1680391/1‑62 (MQ=255) cATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAGCAGTGGCTTAAc  >  1:1400230/1‑62 (MQ=255)                                                  |             CATTAGTGCTGAGTAAATTGCCGGATGACATCAGAACGATGCCATCCGAACAGTGGCTTAAC  >  minE/799853‑799914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑