| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 100,785 | C→T | R352H (CGC→CAC) | hofB ← | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 100,785 | 0 | C | T | 100.0% | 18.3 / NA | 8 | R352H (CGC→CAC) | hofB | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/8); total (0/8) | |||||||||||
CAAAGTTTGCGTACCAGACGCTGGGCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGC > minE/100776‑100836 | cAAAGTTTGTGTACCAGACGCTGGTCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGc < 1:1378974/61‑1 (MQ=255)cAAAGTTTGTGTACCAGACGCTGGGCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGc < 1:1738795/61‑1 (MQ=255)cAAAGTTTGTGTACCAGACGCTGGGCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGc < 1:2176371/61‑1 (MQ=255)cAAAGTTTGTGTACCAGACGCTGGGCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGc < 1:2496978/61‑1 (MQ=255)cAAAGTTTGTGTACCAGACGCTGGGCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGc < 1:2873999/61‑1 (MQ=255)cAAAGTTTGTGTACCAGACGCTGGGCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGc < 1:659921/61‑1 (MQ=255)cAAAGTTTGTGTACCAGACGCTGGGCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGc < 1:808704/61‑1 (MQ=255)cAAAGTTTGTGTACCAGACGCTGGGCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGc < 1:992191/61‑1 (MQ=255) | CAAAGTTTGCGTACCAGACGCTGGGCTATTACCAGCGTAAGCGCCGATGATAGCATCCAGC > minE/100776‑100836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |