Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,013,816	G→A	A155V (GCG→GTG)	fumC ←	fumarate hydratase (fumarase C), aerobic Class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,013,816	0	G	A	100.0%	106.0 / NA	41	A155V (GCG→GTG)	fumC	fumarate hydratase (fumarase C), aerobic Class II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/0);  total (41/0)

CGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCT  >  minE/1013810‑1013871       |                                                        cgcAGCACCGGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2389049/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGtt              >  1:1461687/1‑50 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGt               >  1:2431739/1‑49 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGa           >  1:2840173/1‑53 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:3189805/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2744469/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2762125/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2789298/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2793428/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2807734/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:3062314/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:3171611/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2544158/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:510542/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:554372/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:631544/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:71441/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:716414/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:755628/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:773240/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:933026/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:958859/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1481483/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1139376/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1189274/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1210855/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1284701/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1290371/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:131123/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1454168/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1458389/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1463863/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2640790/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1663758/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:1736484/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2034192/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2101602/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2473235/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:2478422/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:113423/1‑62 (MQ=255) cgcAGCACCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCt  >  1:254493/1‑62 (MQ=255)       |                                                        CGCAGCGCCAGCAGCGCCGCAACGTGCATCGCCGTCGGAAAGACATCGTTGGAACTTTGGCT  >  minE/1013810‑1013871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑