Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,683,488	T→G	T496P (ACC→CCC)	yfhM ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,683,488	0	T	G	100.0%	355.7 / NA	92	T496P (ACC→CCC)	yfhM	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (92/0);  total (92/0)

CGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAA  >  minE/1683477‑1683545            |                                                          cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:283808/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:1780754/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:3098231/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2980003/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:29781/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2943351/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:1965514/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2001597/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2008084/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2880579/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2064958/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2084429/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2864887/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:103638/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2805811/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2104720/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2780712/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2181357/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2778149/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2237151/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2774249/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2340490/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2439005/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2455371/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:2652622/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:107428/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:891623/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:679862/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:1320153/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:1330032/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:1352465/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:1360171/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:136668/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:1071773/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:469948/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:355606/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:33638/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:33349/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:1627123/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcgc                            >  1:1686922/1‑43 (MQ=255) cGTTAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATgcga                            >  1:2939595/1‑42 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCGATGCGCTCTGGCATAAAATCTTCGACg       >  1:1440439/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGct                           >  1:1349944/1‑41 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:967576/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:2539852/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:934728/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:901599/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:644054/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:621235/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:54029/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:2915555/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:512452/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:328341/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:2087461/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:1647468/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:1320030/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:185580/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:190256/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:1911443/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:1929345/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:1547188/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:1407163/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:1478245/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:213296/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:2233005/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:1155354/1‑61 (MQ=255)    tAGCGGGGGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACg       >  1:2524618/1‑61 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATgcg                             >  1:2099146/1‑35 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:565896/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:593635/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:584904/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:1591461/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:1300928/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:780100/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:839382/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:1248275/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:897018/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:1097023/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:1044977/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:1438838/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:1461399/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:382900/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:1508088/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:2529366/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:315735/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:1943053/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:2022137/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:204191/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:2102636/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:2337337/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:2710522/1‑62 (MQ=255)        ggggGTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGaa  >  1:2601020/1‑62 (MQ=255)            |                                                          CGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAA  >  minE/1683477‑1683545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑