Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,818,762	T→G	intergenic (‑21/‑199)	mltB ← / → gutQ	membrane‑bound lytic murein transglycosylase B/predicted phosphosugar‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,818,762	0	T	G	100.0%	76.6 / NA	21	intergenic (‑21/‑199)	mltB/gutQ	membrane‑bound lytic murein transglycosylase B/predicted phosphosugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (0/21);  total (0/21)

CAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTC  >  minE/1818757‑1818816      |                                                       cAGATGCAACCAGTGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:147838/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCTCGCACAAGGAAGCGGTAGTc  <  1:843592/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:2755203/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:9146/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:673884/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:577748/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:483622/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:3195802/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:3169613/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:3126842/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:1166908/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:2737884/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:1901025/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:1867074/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:1652064/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:1531287/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:136842/60‑1 (MQ=255) cAGATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:1347269/60‑1 (MQ=255)  aGATGCAGCCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:171701/59‑1 (MQ=255)   gATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:421108/58‑1 (MQ=255)   gATGCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTc  <  1:614017/58‑1 (MQ=255)      |                                                       CAGATTCAACCAGGGGCAAGTATGGTAAAGCATCACGCCCCGCACAAGGAAGCGGTAGTC  >  minE/1818757‑1818816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑