Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,908,111	T→A	E452V (GAA→GTA)	recC ←	exonuclease V (RecBCD complex), gamma chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,908,111	0	T	A	100.0%	52.6 / NA	18	E452V (GAA→GTA)	recC	exonuclease V (RecBCD complex), gamma chain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/0);  total (18/0)

AGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTA  >  minE/1908096‑1908157                |                                               aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:2750355/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:914020/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:908539/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:805038/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:387116/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:367939/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:2969860/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:296460/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:1200715/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:2629392/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:2524081/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:2282457/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:2002326/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:1928688/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:1785874/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:1520531/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:1494154/1‑62 (MQ=255) aGGCTGATAAACGCTACCAGTACAGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTa  >  1:355590/1‑62 (MQ=255)                |                                               AGGCTGATAAACGCTTCCAGTACCGGATGTGACTGCCGCGCACGACGGTCGGAAATGGCGTA  >  minE/1908096‑1908157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑