Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,356,349 G→A P66S (CCG→TCG)  yihD ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,356,3490GA100.0% 1041.0 / NA 301P66S (CCG→TCG) yihDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (191/110);  total (191/110)

Only 100 of 301 total aligned reads displayed.

ATAATCTTTCTGCAAACCCGGGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATCAG  >  minE/2356329‑2356397
                    |                                                
aTAATCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAAt                           >  1:1492368/1‑44 (MQ=255)
aTAATCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGt            >  1:513054/1‑59 (MQ=255)
aTAATCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGt            >  1:1106591/1‑59 (MQ=255)
aTAATCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGt            >  1:3230548/1‑59 (MQ=255)
aTAATCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGt            >  1:2547044/1‑59 (MQ=255)
aTAATCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGt            >  1:2618896/1‑59 (MQ=255)
aTAATCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGt            >  1:392651/1‑59 (MQ=255)
aTAATCTT‑‑TGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGt            >  1:2756911/1‑57 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:247756/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:2528522/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:1834421/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:333229/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:1921657/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:55419/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:436876/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:2180633/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:2003927/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:2050287/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:2069693/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:2132017/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:713519/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:969423/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:904930/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:820920/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:2631091/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:1180241/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:882743/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:1212279/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:1349496/56‑1 (MQ=255)
  aaTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:1279739/56‑1 (MQ=255)
   aTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:285772/55‑1 (MQ=255)
   aTCTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAgg             <  1:1369043/55‑1 (MQ=255)
   aTCTTTATGCTAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTGAgg             <  1:2444878/55‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:3152647/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:2324619/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:3209649/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:309638/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:1013089/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:527238/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:859632/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:1952181/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:1393902/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:576466/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:1423998/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:632720/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:657735/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:1688857/62‑1 (MQ=255)
     cTTTCTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATc    <  1:1470881/62‑1 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCCCGCATTTTCAGGTGATAGa      >  1:741696/1‑56 (MQ=39)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCAtttt                 >  1:684003/1‑45 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCGGGTGATAGa      >  1:84715/1‑56 (MQ=39)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAgt      >  1:2203881/1‑55 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:3183005/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:588942/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:56845/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:311111/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:486777/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:937919/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:3052388/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:983038/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:2972257/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1976205/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1050879/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1134492/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1306321/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1534351/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1601222/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1630288/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1644306/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1805662/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:1848556/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:191294/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:2230421/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:289085/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:2399885/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:2774137/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGa      >  1:2288458/1‑56 (MQ=255)
         cTGCAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGAAAGa      >  1:186364/1‑56 (MQ=39)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGAt         >  1:3006490/1‑50 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:1702636/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:2944779/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:1075409/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:1130143/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:1205942/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:144877/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:2816322/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:779040/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:2710308/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:696776/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:2693430/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:2470912/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:2393516/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:1927323/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:2915458/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:2110632/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:3277711/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:464017/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:224627/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:2278692/1‑52 (MQ=255)
            cAAACCCGAGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAg       >  1:359116/1‑52 (MQ=255)
                    |                                                
ATAATCTTTCTGCAAACCCGGGATCACCGCATCTTTCGCGGAATCACGCATTTTCAGGTGATAGATCAG  >  minE/2356329‑2356397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑