BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	196,144	G→A	S486F (TCC→TTC)	proS ←	prolyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	196,144	0	G	A	100.0%	479.8 / NA	136	S486F (TCC→TTC)	proS	prolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/111); total (25/111)

Only 100 of 136 total aligned reads displayed.

GCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATA  >  minE/196096‑196191

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:1470136/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:1830611/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:3006738/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:1382653/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:1422860/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:2428470/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:1355159/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:81926/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:3286933/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:3105070/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:2617032/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:1084254/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:266840/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:1797704/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGAATTTGTGCATGt                                     >  1:1048284/1‑61 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTATTGTACGCGGAATAATTTGTGCATGtt                                    >  1:896674/1‑62 (MQ=255)

gCACGCAGTTCGCTGTACAGTTTCTCACCAAGCTCTTGTACGCGGAAGAATTTGTGCATGtt                                    >  1:3187944/1‑62 (MQ=255)

                                  ctTGTACGCTGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2237317/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAGTTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:320898/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAGTTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:317078/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCtata  <  1:2780254/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:3185904/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2712900/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:3236338/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:3171229/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:3140775/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:3039771/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2986885/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:29561/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2936935/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2921054/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:945339/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:624754/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1008557/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:938459/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:898805/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:875960/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:832775/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:826321/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:749404/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:736347/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:735147/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:699283/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:3238002/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:490786/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:470405/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:451675/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:4285/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:422270/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:367925/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:366624/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:338690/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:3282675/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1371941/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2686679/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1944374/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1926453/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1877805/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1781699/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1666755/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1626502/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1586765/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1414997/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2006872/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1361127/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1309357/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1228936/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1206614/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1142487/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1141255/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:112350/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:10948/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1013167/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2673825/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2531303/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2442134/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2384799/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2549761/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2361691/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2079078/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:233380/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2292283/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2278801/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2243130/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:218612/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2167330/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCCCCACCTGGAACGGCGCGATa  <  1:352152/62‑1 (MQ=255)

                                  ctTGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCCCCACCTGGAACGGCGCGATa  <  1:2039309/62‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:3083653/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2774854/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:106071/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2823007/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2708068/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2504882/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2387041/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1401316/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:482449/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:2209250/61‑1 (MQ=255)

                                   ttGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:20465/61‑1 (MQ=255)

                                    tGTACGCGGAAGAATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATa  <  1:1635911/60‑1 (MQ=255)

GCACGCAGTTCGCTGTACAGTTTCTCAGCAAGCTCTTGTACGCGGAAGGATTTGTGCATGTTCATCGGCAGAATCGCCACCTGGAACGGCGCGATA  >  minE/196096‑196191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`