Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,518,129 C→T V316M (GTG→ATG)  spoT ← bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,518,1290CT100.0% 411.0 / NA 118V316M (GTG→ATG) spoTbifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (85/33);  total (85/33)

Only 100 of 118 total aligned reads displayed.

ACACCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCA  >  minE/2518071‑2518158
                                                          |                             
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:3131417/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:1921638/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:1953780/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:2067247/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:2201762/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:2235146/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:2332569/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:2541662/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:2590319/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:2671066/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:2845834/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:1080549/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:351099/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:480435/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:508917/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:628516/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:65649/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:675750/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:787402/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:788952/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:792498/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:947645/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:1383372/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:1773300/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:1248644/62‑1 (MQ=255)
acacCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:1768311/62‑1 (MQ=255)
     cATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:3136560/57‑1 (MQ=255)
                    gTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAgg        >  1:2262365/1‑62 (MQ=255)
                           tCTTCGGTACGGATCTGGACCTCAACCGGCATAcc                            <  1:2455253/35‑1 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACGGTGCGGGCCGATCATCGAGGTGTGCa  >  1:535757/1‑54 (MQ=39)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATTATCGAGGTGTGCa  >  1:2008902/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAgg        >  1:3243597/1‑48 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGc   >  1:1639844/1‑53 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1310392/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:55986/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:547982/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:512969/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3165335/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1314252/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3235029/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:500403/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1364079/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:431701/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:136713/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3278566/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3274835/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3270929/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1158688/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:948366/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1036089/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:931468/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:928111/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:917102/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:821341/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1127929/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1154935/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:572085/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:781821/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:736994/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1164919/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:655671/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:652632/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3063486/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:599608/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:573235/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2100167/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2387932/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2334198/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1548632/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2238887/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1669356/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:218675/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2176846/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2124011/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2429792/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1692190/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2057861/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2028215/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1986949/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1943369/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1891055/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1856243/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1854201/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2506459/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1831362/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3040395/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3016594/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3002877/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2976363/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2958237/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2911256/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1423863/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2818281/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2785645/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:2683118/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1492439/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1537241/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:1537421/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCGGGCCGATCATCGAGGTGTGCa  >  1:3119691/1‑54 (MQ=255)
                                  tACGGATCTGGACCTCAACCGGCATACCGTGCAGGCCGATCATCGAGGTGTGCa  >  1:3099899/1‑54 (MQ=38)
                                                          |                             
ACACCCATCTCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCA  >  minE/2518071‑2518158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑