Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,563,007	G→C	A357G (GCT→GGT)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,563,007	0	G	C	100.0%	82.8 / NA	22	A357G (GCT→GGT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (22/0);  total (22/0)

CACGGCGGCATAGGCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGA  >  minE/2562988‑2563049                    |                                           cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGc                >  1:2638961/1‑48 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCg   >  1:1876348/1‑61 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCg   >  1:2253277/1‑61 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:1514310/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:593245/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:569191/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:494087/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:3246798/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:3166976/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:2845558/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:2517642/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:217544/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:2149607/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:2050583/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:1952313/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:1948660/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:1782138/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:1674515/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:1617482/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:160189/1‑62 (MQ=255) cacGGCGGCATAGGCTGTACCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGa  >  1:1245492/1‑62 (MQ=255) cacGGCGGCATAGGCTGCACCCTCGCTGACCACTGGCGGCATACgg                  >  1:898774/1‑46 (MQ=255)                    |                                           CACGGCGGCATAGGCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGA  >  minE/2562988‑2563049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑