Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,806,946 T→C L320P (CTG→CCG)  tyrB → tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,806,9460TC100.0% 485.5 / NA 124L320P (CTG→CCG) tyrBtyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (90/34);  total (90/34)

Only 100 of 124 total aligned reads displayed.

AAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGG  >  minE/2806901‑2806966
                                             |                    
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGTAATGCGTCAGGaa      >  1:821435/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:211283/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:1624472/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:1657209/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2694862/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2652876/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2630125/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2064171/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:406587/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:1940056/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:3208303/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2577201/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2290119/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2425224/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2498285/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2978242/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:2488343/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:10894/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:1256033/1‑62 (MQ=255)
aaGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGaa      >  1:287770/1‑62 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTTGCATTCCGGCAATGCGTCAGGAATTgg  <  1:2049916/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:2481299/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1093282/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:2768414/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:2413144/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:3142628/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:3150580/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:79478/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1263371/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1337647/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:3285325/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:21860/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:353473/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:354874/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:2103575/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1446045/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:150591/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:494098/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1599759/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:590956/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1972112/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1642409/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1526974/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1877390/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:6201/62‑1 (MQ=255)
    cAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:1579582/62‑1 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGTGTCAGGAATTg   >  1:869577/1‑39 (MQ=37)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGa       >  1:1934386/1‑35 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:964533/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2870400/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2949193/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2850232/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2883347/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:293894/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:3002625/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:3101396/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:3177360/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:486775/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:574854/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:598717/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:671578/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:734679/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:832347/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:844358/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:949428/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1038753/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2078772/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1059006/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1130060/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1347204/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1543370/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1561906/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1569329/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1628858/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1741640/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:17441/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1863238/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:1924461/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2051766/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2060347/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2072810/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2821450/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:248216/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2810078/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:276450/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2722434/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2586505/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2584364/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2572514/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:248571/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2135481/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2414586/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2407845/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2310664/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2281422/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:227476/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:224754/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTg   >  1:2175738/1‑39 (MQ=255)
                          gagaTGCGTACTCGCATTCCGGCAATGCGT‑AGGAATTg   >  1:1995832/1‑38 (MQ=25)
                             aTGCGTACTCGCATTCCGGCAATGCGTCAGGAATTgg  <  1:931145/37‑1 (MQ=255)
                                             |                    
AAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGG  >  minE/2806901‑2806966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑