Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,960,381	T→G	L204R (CTG→CGG)	yjjV →	predicted DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,960,381	0	T	G	100.0%	40.6 / NA	12	L204R (CTG→CGG)	yjjV	predicted DNase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/0);  total (12/0)

CAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACG  >  minE/2960331‑2960392                                                   |            cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:15061/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:1947762/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:2128535/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:2315097/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:2508881/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:2585199/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:2707981/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:3020551/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:407218/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:547293/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtta  >  1:741909/1‑59 (MQ=255) cAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCGGTAAACCgtt   >  1:251214/1‑59 (MQ=255)                                                   |            CAGTAAAACCCGCGATGTCATCGCAAAATTACCGCTGGCATCGTTATTGCTGGAAACCGACG  >  minE/2960331‑2960392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑