Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 301,836 C→A V134V (GTC→GTA)  amtB → ammonium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE301,8360CA86.7% 270.4 / 54.3 120V134V (GTC→GTA) amtBammonium transporter
Reads supporting (aligned to +/- strand):  ref base C (16/0);  new base A (104/0);  total (120/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 120 total aligned reads displayed.

TATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGC  >  minE/301786‑301880
                                                  |                                            
taacAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1050229/4‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCATGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1873637/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGTGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:345804/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAgg                                            >  1:2276698/1‑53 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAgg                                   >  1:1702953/1‑61 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAg                                    >  1:901784/1‑61 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:279811/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:3225241/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:3177029/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:3131043/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:3004826/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:298790/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:296356/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2908281/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2889557/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2853459/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2367091/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2728765/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2691653/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2691123/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2628888/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2616483/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2572310/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2571104/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:239233/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2529960/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:746328/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:965821/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:941824/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:934565/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:898865/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:89195/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:882662/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:871868/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:775935/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:757865/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:756742/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:3290133/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:569880/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:540178/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:500419/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:436089/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:407001/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:396509/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:388306/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:359760/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:3296384/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1376725/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1759264/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1755790/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1705322/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1668377/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1639369/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1556734/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:153275/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:152542/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1516758/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:149774/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2287966/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1369943/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1354436/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1326709/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:128430/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1230497/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1229682/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1168523/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1098505/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1061323/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1050796/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1974854/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2184604/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2207435/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2112528/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2213729/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2239176/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2084898/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2046663/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1926016/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:2300966/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1885459/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1813730/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1797102/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:1763273/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGATAGt                                   >  1:17848/1‑62 (MQ=255)
tATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTAGGCTTGAAAGt                                   >  1:3290944/1‑62 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTTGGGCGCTGGCGGAACGAATCCGCTTCTCAg   >  1:1017526/1‑61 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCg           >  1:206718/1‑53 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCg           >  1:1812789/1‑53 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAg   >  1:1690553/1‑61 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAg   >  1:2208655/1‑61 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAg   >  1:889344/1‑61 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGc  >  1:1049026/1‑62 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGc  >  1:850710/1‑62 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGc  >  1:2656764/1‑62 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGc  >  1:1373623/1‑62 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGc  >  1:713702/1‑62 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGc  >  1:583107/1‑62 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGc  >  1:400391/1‑62 (MQ=255)
                                 tttGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGc  >  1:311518/1‑62 (MQ=255)
                                 tttGCCTGCATTACCGTCGCCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAg   >  1:2665898/1‑61 (MQ=255)
                                                  |                                            
TATCAGTATATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGATAGTTGGGGCGCTGGCGGAACGAATCCGCTTCTCAGC  >  minE/301786‑301880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑