Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	339,261	A→G	G196G (GGT→GGC)	copA ←	copper transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	339,261	0	A	G	100.0%	207.7 / NA	55	G196G (GGT→GGC)	copA	copper transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (55/0);  total (55/0)

TCATGTTATCGCCGATCATCCCCCAGACCATCACCGGGATACCCACCGCCAGTGCGACAATTG  >  minE/339221‑339283                                         |                       tcatGTTATCGCCGATCATCCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAAtt   >  1:699867/1‑62 (MQ=255) tcatGTTATCGCCGATCATCCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAAtt   >  1:3059319/1‑62 (MQ=255) gcatGTTATCGCCGATCATCCCCCAGACCCTCACCGGGATGcc                      >  1:749754/2‑43 (MQ=38)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTTCGACAATtg  >  1:2103352/1‑46 (MQ=39)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACa      >  1:14164/1‑42 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAAtt   >  1:3125727/1‑45 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:3272711/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2833671/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2902580/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2909005/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2910935/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2937816/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2963496/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:3167168/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:3225784/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:3243034/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:3267262/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:3267515/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:260798/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:345817/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:397495/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:457917/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:55072/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:597162/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:632690/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:802453/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:830544/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:836345/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:917877/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1582304/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1080876/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:117767/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1271626/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1317011/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1328993/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1332769/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1399093/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1427569/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1442326/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1515391/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1527926/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1538935/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2758120/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1645395/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1669287/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1779992/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1791094/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2105957/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2108364/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2162995/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2204355/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:230120/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2316799/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:2404468/1‑46 (MQ=255)                  atcCCCCAGACCATCACCGGGATGCCCACCGCCAGTGCGACAATtg  >  1:1059934/1‑46 (MQ=255)                                         |                       TCATGTTATCGCCGATCATCCCCCAGACCATCACCGGGATACCCACCGCCAGTGCGACAATTG  >  minE/339221‑339283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑