Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	350,093	A→G	K199K (AAA→AAG)	hyi →	hydroxypyruvate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	350,093	0	A	G	100.0%	140.3 / NA	37	K199K (AAA→AAG)	hyi	hydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/22);  total (15/22)

GGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGG  >  minE/350051‑350146                                           |                                                      ggACGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:1911584/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:1320208/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:978580/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:750865/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:658193/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:533698/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:37196/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:341596/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:3209776/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:3079354/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:2656936/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:2460197/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:2403875/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:1104312/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:13611/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:2213230/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:1690169/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:1707496/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:2074253/62‑1 (MQ=255) ggAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:1883400/62‑1 (MQ=255)  gAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:2446066/61‑1 (MQ=255)       tGAATTAACCAACACCATGACTCAGTGGGCTGATAAGATTGGTCACCTGCAAATTg                                    <  1:82388/56‑1 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:3141321/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:2903472/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:287178/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:43835/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:446056/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:478410/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:2628967/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:534787/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:574810/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:1361059/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:742580/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:1335446/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:866432/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:867342/1‑61 (MQ=255)                                    cTGATAAGATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg  >  1:2374119/1‑61 (MQ=255)                                           |                                                      GGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGG  >  minE/350051‑350146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑