| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,954,781 | Δ1 bp | intergenic (‑69/‑99) | ygfB ← / → zapA | hypothetical protein/protein that localizes to the cytokinetic ring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,954,778 | 0 | A | . | 98.1% | 226.9 / ‑1.4 | 52 | intergenic (‑66/‑102) | ygfB/zapA | hypothetical protein/protein that localizes to the cytokinetic ring |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (23/28); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
GACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAA > minE/1954708‑1954843 | gACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGATCAGa < 1:1480960/71‑1 (MQ=255)gACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGa < 1:1198127/71‑1 (MQ=255)gACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGa < 1:494125/71‑1 (MQ=255) aCATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGa < 1:38373/70‑1 (MQ=255) aGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGAAAggg < 1:3184963/70‑1 (MQ=255) ttCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGt < 1:1605006/71‑1 (MQ=255) tcgtcAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGtct < 1:189370/69‑1 (MQ=255) gtcAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCtcttc > 1:1692884/1‑71 (MQ=255) tcAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCtcttct > 1:818812/1‑71 (MQ=255) aaaGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGt < 1:780063/62‑1 (MQ=255) atatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGc > 1:1311402/1‑71 (MQ=255) atatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGc > 1:1587034/1‑71 (MQ=255) atatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGc > 1:201860/1‑71 (MQ=255) tatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGtc > 1:526734/1‑52 (MQ=255) tatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGc > 1:55309/1‑70 (MQ=255) tatTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCt < 1:1521982/71‑1 (MQ=255) atTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCta > 1:271759/1‑71 (MQ=255) atTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCta > 1:35077/1‑71 (MQ=255) atTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCta > 1:724894/1‑71 (MQ=255) ttCATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCta < 1:1685112/70‑1 (MQ=255) cATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAg > 1:572432/1‑71 (MQ=255) cATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAg > 1:1458707/1‑71 (MQ=255) cATGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAg > 1:847866/1‑71 (MQ=255) tGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAgtg < 1:3001712/71‑1 (MQ=255) tGATATGCTACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAgtg > 1:2396308/1‑71 (MQ=255) tACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCtctt < 1:3134247/42‑1 (MQ=255) tACCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCtctt < 1:3172603/42‑1 (MQ=255) aCCACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCtt > 1:2763911/1‑71 (MQ=255) ccACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCt < 1:3049316/69‑1 (MQ=255) cACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCg < 1:610674/71‑1 (MQ=255) cACTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCg > 1:637766/1‑71 (MQ=255) aCTTTGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGc < 1:2292204/71‑1 (MQ=255) tttGGGCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCg > 1:2947152/1‑68 (MQ=255) gCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGAcc < 1:123557/69‑1 (MQ=255) gCCCTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCAt < 1:355198/71‑1 (MQ=255) ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGt < 1:939134/40‑1 (MQ=255) ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCtat < 1:3023604/46‑1 (MQ=255) ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCtat < 1:994595/46‑1 (MQ=255) ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCtat < 1:1561526/46‑1 (MQ=255) ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATgg < 1:2251848/71‑1 (MQ=255) ccTGGTGGACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATgg > 1:2054687/1‑71 (MQ=255) tggtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGc < 1:2678884/60‑1 (MQ=255) gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAgtg > 1:501888/1‑46 (MQ=255) gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGt < 1:2786317/69‑1 (MQ=255) gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGt < 1:1866939/69‑1 (MQ=255) gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGt < 1:646542/69‑1 (MQ=255) gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTc > 1:2225680/1‑70 (MQ=255) gtggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTc > 1:2302270/1‑70 (MQ=255) tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGt > 1:1300626/1‑35 (MQ=255) tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:586973/71‑1 (MQ=255) tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:557496/71‑1 (MQ=255) tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:225327/71‑1 (MQ=255) tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:1408664/71‑1 (MQ=255) tggACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa < 1:485620/71‑1 (MQ=255) gACCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTaaa < 1:1972610/71‑1 (MQ=255) aCCAG‑AAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTa > 1:464536/1‑68 (MQ=255) | GACATAAGCATTCGTCGTCAAAGGGAGGAATATTCATGATATGCTACCACTTTGGGCCCTGGTGGACCAGAAAAGGGCTTGTCTCTTCTCATCAGGGTAGCTATAGTGTCGCCCCTTCGCAGACCATGGGTCTAAA > minE/1954708‑1954843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |