Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,892,019 +GC intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,892,0191.G100.0% 141.4 / NA 39intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/17);  total (22/17)
*minE1,892,0192.C100.0% 141.8 / NA 39intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/17);  total (22/17)

GGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTT  >  minE/1891953‑1892074
                                                                   ||                                                       
ggCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                        >  1:1104619/1‑67 (MQ=255)
         cTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGaa                                              >  1:2693073/1‑71 (MQ=255)
          tGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                             >  1:1736054/1‑71 (MQ=255)
           gAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                            >  1:1805179/1‑71 (MQ=255)
            aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg                                           >  1:2643285/1‑71 (MQ=255)
              aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCggg                                         <  1:104353/71‑1 (MQ=255)
              aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCggg                                         <  1:283767/71‑1 (MQ=255)
              aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCggg                                         >  1:1403373/1‑71 (MQ=255)
                  ttcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCgg                                     <  1:761040/71‑1 (MQ=255)
                   tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                    >  1:2372493/1‑71 (MQ=255)
                   tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                    >  1:2098701/1‑71 (MQ=255)
                       ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                                <  1:1087883/71‑1 (MQ=255)
                           gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                  >  1:2568811/1‑65 (MQ=255)
                           gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                            >  1:1007063/1‑71 (MQ=255)
                           gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                            <  1:2704129/71‑1 (MQ=255)
                               cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                        <  1:118733/71‑1 (MQ=255)
                               cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                        <  1:2703952/71‑1 (MQ=255)
                                 cTAAAACGACATATACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGCTCgca                                    >  1:768793/1‑55 (MQ=255)
                                   aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                               <  1:552532/44‑1 (MQ=255)
                                    aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                    >  1:1729490/1‑70 (MQ=255)
                                     aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                             >  1:1049059/1‑44 (MQ=255)
                                     aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                             >  1:2964682/1‑44 (MQ=255)
                                     aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                             >  1:2089490/1‑44 (MQ=255)
                                     aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                             >  1:2832960/1‑44 (MQ=255)
                                     aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                             >  1:115711/1‑44 (MQ=255)
                                     aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCa                  <  1:1203109/71‑1 (MQ=255)
                                        gACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATcc               <  1:3040429/71‑1 (MQ=255)
                                         aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTc                                       <  1:661806/46‑1 (MQ=255)
                                         aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg              <  1:2655337/71‑1 (MQ=255)
                                         aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg              <  1:1198237/71‑1 (MQ=255)
                                              tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcg                         >  1:2383540/1‑55 (MQ=255)
                                               aCCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                    >  1:1926592/1‑59 (MQ=255)
                                                ccGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa        <  1:922146/70‑1 (MQ=38)
                                                 cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGAtt      <  1:1880038/71‑1 (MQ=255)
                                                  gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc                <  1:1747877/60‑1 (MQ=255)
                                                   ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAt                 >  1:1166264/1‑58 (MQ=255)
                                                   ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAt                 >  1:1972528/1‑58 (MQ=255)
                                                   ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAt                 >  1:305442/1‑58 (MQ=255)
                                                      gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt  <  1:393256/70‑1 (MQ=38)
                                                         gAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                      >  1:460775/1‑47 (MQ=37)
                                                                   ||                                                       
GGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTT  >  minE/1891953‑1892074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: