| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,763,636 | 0 | A | G | 21.9% | 57.9 / 14.4 | 32 | noncoding (208/1542 nt) | rrsG | 16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (12/13); new base G (4/3); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAT > minE/1763571‑1763705 | tGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTcc > 1:1440639/1‑71 (MQ=255) ggTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTccc > 1:1694180/1‑71 (MQ=255) gTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGATGCCCGAGGGTccc < 1:1380256/70‑1 (MQ=255) aGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCctc > 1:256722/1‑71 (MQ=255) ccGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCtt > 1:227598/1‑71 (MQ=255) cGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCttt < 1:752168/71‑1 (MQ=39) cGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCtt < 1:475515/70‑1 (MQ=40) tACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGt > 1:1979190/1‑71 (MQ=39) ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGTCCCCCTCTTTGGTc < 1:400846/70‑1 (MQ=255) ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCt < 1:2188607/71‑1 (MQ=39) ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCt > 1:1110209/1‑71 (MQ=39) ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCt > 1:1311223/1‑71 (MQ=39) ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCt < 1:1430830/71‑1 (MQ=39) ccccACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCt < 1:1427045/71‑1 (MQ=39) caccaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGc > 1:1822044/1‑71 (MQ=39) accaACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAGGGGCCCCCTCTTTGGTCTTGCg < 1:1754755/71‑1 (MQ=255) aGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTAt > 1:680475/1‑71 (MQ=34) tGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTa > 1:1912397/1‑64 (MQ=35) tGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTa > 1:255925/1‑64 (MQ=35) gggCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTAc > 1:2492912/1‑71 (MQ=34) ggCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCt < 1:2152553/68‑1 (MQ=35) ggCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTa < 1:690454/69‑1 (MQ=35) aTCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTc < 1:2367888/71‑1 (MQ=34) cAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTa < 1:2680425/71‑1 (MQ=34) aaGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCg > 1:2854034/1‑44 (MQ=38) gagGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGc < 1:1115698/41‑1 (MQ=38) gCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTa > 1:659568/1‑49 (MQ=38) gCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTa > 1:213967/1‑49 (MQ=38) gCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCag < 1:1614129/59‑1 (MQ=35) ccGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCACTAGTTATCCCCCTc < 1:2976372/71‑1 (MQ=255) cGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTcc < 1:2542788/71‑1 (MQ=34) gAAGGTCCCCCTCTTTGGTCTTGCGACATTATGTGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCa > 1:1942451/1‑71 (MQ=21) aaGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAt > 1:576395/1‑71 (MQ=34) | TGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCAT > minE/1763571‑1763705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |