Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	13,094	A→T	D311V (GAT→GTT)	dnaK →	chaperone Hsp70, co‑chaperone with DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	13,094	0	A	T	100.0%	162.3 / NA	50	D311V (GAT→GTT)	dnaK	chaperone Hsp70, co‑chaperone with DnaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/24);  total (26/24)

AGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGA  >  minE/13026‑13160                                                                     |                                                                   aGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTc                                                                  <  1:165131/71‑1 (MQ=255)    cgcgACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTgg                                                               <  1:575161/71‑1 (MQ=255)     gcgACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTgg                                                               >  1:2692332/1‑70 (MQ=255)      cgACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTa                                                             <  1:634248/71‑1 (MQ=255)       gACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTa                                                             <  1:2058839/70‑1 (MQ=255)        aCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTaaa                                                           >  1:2242298/1‑71 (MQ=255)        aCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTaaa                                                           <  1:1873498/71‑1 (MQ=255)         ccGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAAc                                                          >  1:1098994/1‑71 (MQ=255)         ccGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAAc                                                          >  1:1597174/1‑71 (MQ=255)              ccGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTa                                                             <  1:2259460/63‑1 (MQ=255)                 aaaCACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCAt                                                  <  1:924128/71‑1 (MQ=255)                 aaaCACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCAt                                                  >  1:1460306/1‑71 (MQ=255)                   acacATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTg                                                <  1:1739081/71‑1 (MQ=255)                   acacATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTg                                                <  1:2258422/71‑1 (MQ=255)                     acaTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAg                                              >  1:2090123/1‑71 (MQ=255)                       aTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGcc                                            >  1:2334058/1‑71 (MQ=255)                       aTGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGcc                                            >  1:1449055/1‑71 (MQ=255)                           aCATCAAAGTGACTCGTGCGAATCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTg                                        <  1:2907572/71‑1 (MQ=255)                             aTCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTcc                                                    <  1:1536481/57‑1 (MQ=255)                             aTCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGaa                                      <  1:960358/71‑1 (MQ=255)                                aaaGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTg                                                                >  1:1513981/1‑42 (MQ=255)                                  aGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTg                                 >  1:170900/1‑71 (MQ=255)                                   gTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTg                                        <  1:2123015/63‑1 (MQ=255)                                   gTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTg                                        <  1:1178038/63‑1 (MQ=255)                                   gTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTg                                        <  1:1203954/63‑1 (MQ=255)                                   gTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTg                                        <  1:3147999/63‑1 (MQ=255)                                   gTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTg                                        <  1:1834557/63‑1 (MQ=255)                                   gTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGc                                <  1:3062125/71‑1 (MQ=255)                                     gACTCGTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTg                                        <  1:2319753/61‑1 (MQ=255)                                          gTGCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAg                         >  1:1600911/1‑71 (MQ=255)                                            gCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGa                       >  1:2040/1‑71 (MQ=255)                                            gCGAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGa                       >  1:1557614/1‑71 (MQ=255)                                              gAAACTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACg                     <  1:271883/71‑1 (MQ=255)                                               aaaCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGc                           >  1:1040539/1‑64 (MQ=255)                                               aaaCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGc                    >  1:2254425/1‑71 (MQ=255)                                               aaaCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGc                    >  1:1473638/1‑71 (MQ=255)                                                aaCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCt                   <  1:2319693/71‑1 (MQ=255)                                                aaCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCt                   <  1:322043/71‑1 (MQ=255)                                                aaCTGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCt                   <  1:419644/71‑1 (MQ=255)                                                   tGGAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCAt                                                  <  1:2492561/37‑1 (MQ=255)                                                     gAAAGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAg                         >  1:1851576/1‑60 (MQ=255)                                                      aaaGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTg             >  1:1105932/1‑71 (MQ=255)                                                       aaGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTgg                 >  1:2765673/1‑66 (MQ=255)                                                       aaGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTgg                 >  1:861058/1‑66 (MQ=255)                                                       aaGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGt            >  1:2860260/1‑71 (MQ=255)                                                        aGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAg                                    >  1:1023645/1‑46 (MQ=255)                                                        aGCCTGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTc           >  1:628579/1‑71 (MQ=255)                                                            tGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTa       >  1:282485/1‑71 (MQ=255)                                                            tGGTTGAAGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTa       >  1:143862/1‑71 (MQ=255)                                                                  aaGTTCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGa  >  1:2232784/1‑70 (MQ=255)                                                                     |                                                                   AGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGA  >  minE/13026‑13160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑