Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	421,280	G→A	A400V (GCA→GTA)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	421,280	0	G	A	100.0%	186.5 / NA	55	A400V (GCA→GTA)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/25);  total (30/25)

TGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGC  >  minE/421225‑421341                                                        |                                                              tgGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAgg                                                >  1:1351183/1‑71 (MQ=255) tgGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAgg                                                >  1:2206908/1‑71 (MQ=255)           cGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGACCGCGTACTTCCACGCCCCAGGCTGACATCGc                                      >  1:2343719/1‑71 (MQ=255)            gCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCt                                     <  1:181198/71‑1 (MQ=255)               tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCt                                              <  1:738309/59‑1 (MQ=255)               tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCt                                              <  1:1234094/59‑1 (MQ=255)               tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg                                  <  1:2984549/71‑1 (MQ=255)               tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg                                  <  1:1383936/71‑1 (MQ=255)               tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg                                  <  1:819425/71‑1 (MQ=255)               tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg                                  <  1:2351378/71‑1 (MQ=255)                 gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTg                                             >  1:476223/1‑58 (MQ=255)                 gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt                                >  1:1606122/1‑71 (MQ=255)                 gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgt                                 <  1:1730495/70‑1 (MQ=255)                   cacaTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTgg                              <  1:2061554/71‑1 (MQ=255)                      aTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCt                                              <  1:1578887/52‑1 (MQ=255)                        cAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgc                         >  1:1718886/1‑71 (MQ=255)                           ggAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGc                                                      <  1:918592/39‑1 (MQ=255)                           ggAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGc                                                      <  1:403464/39‑1 (MQ=255)                           ggAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTatgt                                 >  1:751656/1‑60 (MQ=255)                            gAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCt                                              >  1:949580/1‑46 (MQ=255)                             aaTTTTTTGTCGAGGAACGGAACGCGTACTTCCACGccc                                                    >  1:2588396/1‑39 (MQ=255)                             aaTTTTTTGTCGAGGAACGGAACGCGTACTTCCACGccc                                                    >  1:338820/1‑39 (MQ=255)                              aTTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAg                   <  1:2472107/71‑1 (MQ=255)                              aTTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAg                   <  1:2877410/71‑1 (MQ=255)                                tttttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgcg                        >  1:782488/1‑64 (MQ=255)                                 ttttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTca                >  1:1754206/1‑71 (MQ=255)                                 ttttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTca                >  1:2316026/1‑71 (MQ=255)                                  tttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCtt                                    >  1:2913622/1‑50 (MQ=255)                                   ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGTACGCGCGCAGt                  >  1:1373950/1‑67 (MQ=255)                                   ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgc                         >  1:77561/1‑60 (MQ=255)                                   ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgc                         >  1:1453560/1‑60 (MQ=255)                                   ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgc                         >  1:1648691/1‑60 (MQ=255)                                   ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGt                  >  1:1071495/1‑67 (MQ=255)                                   ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGt                  >  1:2586233/1‑67 (MQ=255)                                    tGTCGAGGAACGGAACGCGTACTTCCACGCCCCAgg                                                >  1:1755888/1‑36 (MQ=255)                                    tGTCGAGGAACGGAACGCGTACTTCCACGCCCCAgg                                                >  1:1116812/1‑36 (MQ=255)                                    tGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTcata              <  1:2456893/70‑1 (MQ=255)                                    tGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTcata              <  1:2795112/70‑1 (MQ=255)                                     gTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCt                                     <  1:1524285/46‑1 (MQ=255)                                     gTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCt                                     <  1:2089706/46‑1 (MQ=255)                                     gTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCt                                     <  1:1611983/46‑1 (MQ=255)                                      tCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTcataca            <  1:1969336/70‑1 (MQ=255)                                       cGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCttt                                   >  1:1446761/1‑46 (MQ=255)                                        gAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACatat         <  1:1004743/71‑1 (MQ=255)                                          ggaacggaacGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATg        >  1:2346915/1‑70 (MQ=255)                                          ggaacggaacGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGc       <  1:536999/71‑1 (MQ=255)                                          ggaacggaacGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGc       <  1:530269/71‑1 (MQ=255)                                          ggaacggaacGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGc       <  1:2481272/71‑1 (MQ=255)                                           gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt                                >  1:635396/1‑45 (MQ=255)                                           gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt                                >  1:2806027/1‑45 (MQ=255)                                           gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt                                >  1:976398/1‑45 (MQ=255)                                           gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATg        >  1:3039536/1‑69 (MQ=255)                                           gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGc       <  1:2619272/70‑1 (MQ=255)                                                 aacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGc  >  1:1444144/1‑69 (MQ=255)                                                     cgTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCa                    >  1:1627796/1‑47 (MQ=255)                                                        |                                                              TGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGC  >  minE/421225‑421341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑