Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	530,638	T→C	E105E (GAA→GAG)	ybhP ←	predicted DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	530,638	0	T	C	100.0%	353.1 / NA	95	E105E (GAA→GAG)	ybhP	predicted DNase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/44);  total (51/44)

ACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGTTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGTATACGGCATTGC  >  minE/530570‑530699                                                                     |                                                              acaATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTc                                                             <  1:365803/71‑1 (MQ=255)  caATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTc                                                             >  1:950560/1‑70 (MQ=255)   aaTGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCaa                                                           >  1:2916628/1‑71 (MQ=255)     tGCGGCAGTAGAGCACGCTGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATg                                                         >  1:2299668/1‑71 (MQ=255)     tGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATg                                                         >  1:1787199/1‑71 (MQ=255)     tGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATg                                                         <  1:561822/71‑1 (MQ=255)     tGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATg                                                         >  1:2895242/1‑71 (MQ=255)     tGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATg                                                         >  1:1899491/1‑71 (MQ=255)     tGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATg                                                         >  1:1405748/1‑71 (MQ=255)     tGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATg                                                         >  1:2970803/1‑71 (MQ=255)     tGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATg                                                         >  1:143442/1‑71 (MQ=255)        ggCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGa                                                      >  1:1028469/1‑71 (MQ=255)         gCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTTAATGGGAt                                                     <  1:1481841/71‑1 (MQ=255)           aGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATaa                                                   <  1:1329717/71‑1 (MQ=255)           aGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATaa                                                   <  1:875252/71‑1 (MQ=255)             tAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAAcg                                                 >  1:541102/1‑71 (MQ=255)                     gccgcGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTa                                         <  1:2199605/71‑1 (MQ=255)                     gccgcGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTa                                         <  1:1547591/71‑1 (MQ=255)                      ccgcGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTAc                                        >  1:861568/1‑71 (MQ=255)                       cgcgCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACg                                       <  1:1799362/71‑1 (MQ=255)                          gcTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGAt                                                     <  1:45775/54‑1 (MQ=255)                          gcTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGAt                                                     <  1:2513767/54‑1 (MQ=255)                          gcTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGAt                                                     <  1:850161/54‑1 (MQ=255)                          gcTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGAt                                                     <  1:1442354/54‑1 (MQ=255)                          gcTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGAt                                                     <  1:872834/54‑1 (MQ=255)                           cTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGAt                                                     <  1:3135566/53‑1 (MQ=255)                            ttttCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGGGACAGt                                          <  1:3782/63‑1 (MQ=255)                              ttCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  <  1:3120478/69‑1 (MQ=255)                              ttCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTg                                 >  1:611735/1‑70 (MQ=255)                              ttCCGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGc                                >  1:994276/1‑71 (MQ=255)                                ccGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCg                                    >  1:1342595/1‑65 (MQ=255)                                ccGCACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCg                              >  1:3153713/1‑71 (MQ=255)                                 cGCACCATCGACCGAAACATCGCGATTCTCATAATCCTCAATGGGATAACGCGACAGTACGGCGTTGCCGt                             <  1:1284782/71‑1 (MQ=255)                                   cACCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAg                                           <  1:1128057/55‑1 (MQ=255)                                    aCCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGa                           >  1:1729586/1‑70 (MQ=255)                                    aCCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGa                           <  1:578322/70‑1 (MQ=255)                                    aCCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGAt                          <  1:780742/71‑1 (MQ=255)                                    aCCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGAt                          >  1:2777205/1‑71 (MQ=255)                                    aCCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGAt                          <  1:2724432/71‑1 (MQ=255)                                    aCCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGAt                          >  1:2735595/1‑71 (MQ=255)                                    aCCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGAt                          >  1:2133446/1‑71 (MQ=255)                                    aCCATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGAt                          >  1:2352578/1‑71 (MQ=255)                                     ccATCGACCGAAACATCGCGATTCTCCTAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATg                         >  1:22861/1‑71 (MQ=255)                                     ccATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACa                                            >  1:1908447/1‑52 (MQ=255)                                     ccATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACa                                            >  1:2761326/1‑52 (MQ=255)                                     ccATCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATg                         >  1:1040720/1‑71 (MQ=255)                                        tCGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGccc                      >  1:3164613/1‑71 (MQ=255)                                         cGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGt                                   >  1:2356150/1‑57 (MQ=255)                                         cGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCt                     >  1:2486281/1‑71 (MQ=255)                                         cGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCt                     >  1:114283/1‑71 (MQ=255)                                         cGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCt                     >  1:829130/1‑71 (MQ=255)                                         cGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCt                     >  1:312078/1‑71 (MQ=255)                                         cGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCt                     >  1:2323926/1‑71 (MQ=255)                                         cGACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCt                     >  1:2489971/1‑71 (MQ=255)                                          gACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCtt                    >  1:2680920/1‑71 (MQ=255)                                          gACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCtt                    >  1:2117688/1‑71 (MQ=255)                                          gACCGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCtt                    >  1:303744/1‑71 (MQ=255)                                            ccGAAACATCGCGATTCTCATAATGCTCAATGGGATAAcgc                                                <  1:294919/41‑1 (MQ=255)                                            ccGAAACATCGCGATTCTCATAATGCTCAATGGGATAAcgc                                                <  1:2879487/41‑1 (MQ=255)                                            ccGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTcc                  >  1:190962/1‑71 (MQ=255)                                            ccGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTcc                  <  1:1099328/71‑1 (MQ=255)                                             cGAAACATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTcc                  <  1:841930/70‑1 (MQ=255)                                             cGAAACATCGCGATTCTCATAATGCTCAAAGGGATAACGCGACAGTACGGc                                     >  1:1411288/1‑51 (MQ=255)                                                aaCATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCggg               >  1:429508/1‑70 (MQ=255)                                                 aCATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCggg               >  1:263050/1‑69 (MQ=255)                                                  cATCGCGATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGta             >  1:272989/1‑70 (MQ=255)                                                      gcgATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGTATACgg        <  1:945919/71‑1 (MQ=255)                                                       cgATTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGt                             <  1:1481274/49‑1 (MQ=255)                                                         aTTCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGTATACGGCAt     <  1:1786353/71‑1 (MQ=255)                                                          ttCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGt                             <  1:9151/46‑1 (MQ=255)                                                          ttCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGt                             <  1:1939571/46‑1 (MQ=255)                                                          ttCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCgg                <  1:577964/59‑1 (MQ=255)                                                          ttCTCATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGTATACGGCAt     <  1:3137431/70‑1 (MQ=255)                                                           tctcATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTc                   <  1:1494922/55‑1 (MQ=255)                                                           tctcATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTc                   <  1:1987855/55‑1 (MQ=255)                                                           tctcATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTc                   <  1:1118449/55‑1 (MQ=255)                                                           tctcATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTc                   <  1:584803/55‑1 (MQ=255)                                                           tctcATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGTATACGGCAt     <  1:2757916/69‑1 (MQ=255)                                                            ctcATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGTATACGGCATTg   <  1:3020161/70‑1 (MQ=255)                                                            ctcATAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGTATACGGCATTg   <  1:3016451/70‑1 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:1823946/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:2758267/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:80919/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:358048/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:324232/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:2437148/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:194456/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:2586870/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:522594/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGtt                                  >  1:422258/1‑36 (MQ=255)                                                               aTAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGTATACGGCATTGc  <  1:970544/68‑1 (MQ=255)                                                                tAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGt                             <  1:2895596/40‑1 (MQ=255)                                                                tAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGt                             <  1:1675974/40‑1 (MQ=255)                                                                tAATGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGt                             <  1:434741/40‑1 (MQ=255)                                                                 aaTGCTCAATGGGATAACGCGACAGTACGGCGTTGCCGt                             <  1:1913144/39‑1 (MQ=255)                                                                     |                                                              ACAATGCGGCAGTAGAGCACGCCGCGCTTTTCCGCACCATCGACCGAAACATCGCGATTCTCATAATGTTCAATGGGATAACGCGACAGTACGGCGTTGCCGTGATGCCCTTCCGGGTATACGGCATTGC  >  minE/530570‑530699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑