Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	709,001	A→G	Y102H (TAT→CAT)	yceI ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	709,001	0	A	G	100.0%	254.0 / NA	68	Y102H (TAT→CAT)	yceI	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/46);  total (22/46)

CGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATATTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGATTAGTATCGACG  >  minE/708935‑709068                                                                   |                                                                    cGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGtttt                                                                 >  1:2076700/1‑71 (MQ=255)  gCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTg                                                                <  1:1378781/71‑1 (MQ=255)    cGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCg                                                              >  1:3151845/1‑71 (MQ=255)       tAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTa                                                           >  1:1128635/1‑71 (MQ=255)          tatCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         >  1:3093510/1‑70 (MQ=255)           atCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         >  1:491625/1‑69 (MQ=255)              cAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:2702690/70‑1 (MQ=255)              cAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:1117282/70‑1 (MQ=255)               aGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTTTTGAgg                                                     <  1:3278/69‑1 (MQ=255)               aGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:125330/69‑1 (MQ=255)               aGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   <  1:1890388/71‑1 (MQ=255)                 ttCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAAt                                                 >  1:2757291/1‑71 (MQ=255)                  tCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:608713/66‑1 (MQ=255)                  tCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:1980812/66‑1 (MQ=255)                  tCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAgg                                                     <  1:2808237/66‑1 (MQ=255)                   cGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATc                                                <  1:2904468/70‑1 (MQ=255)                   cGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATc                                                <  1:787696/70‑1 (MQ=255)                   cGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATc                                                <  1:2976140/70‑1 (MQ=255)                   cGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATc                                                <  1:1698504/70‑1 (MQ=255)                    gTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   >  1:2381442/1‑66 (MQ=255)                    gTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   >  1:2475962/1‑66 (MQ=255)                    gTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTg                                              <  1:1695872/71‑1 (MQ=255)                        ccGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACt                                          <  1:1615261/71‑1 (MQ=255)                        ccGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACt                                          <  1:1280934/71‑1 (MQ=255)                         cGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGtt                                                                   <  1:2967728/45‑1 (MQ=255)                          gTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGtt                                                                   <  1:1055135/44‑1 (MQ=255)                           tctttcttCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   <  1:1193364/59‑1 (MQ=255)                           tctttcttCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGaa                                                   <  1:692658/59‑1 (MQ=255)                             tttcttCACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCg                                       >  1:163549/1‑69 (MQ=255)                               tcttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGa                                      <  1:823132/68‑1 (MQ=255)                               tcttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAg                                    <  1:590815/70‑1 (MQ=255)                                cttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGAt                                  >  1:181934/1‑71 (MQ=255)                                cttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGAt                                  >  1:706418/1‑71 (MQ=255)                                 ttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGa                                                       <  1:1328724/49‑1 (MQ=255)                                 ttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGa                                                       <  1:475655/49‑1 (MQ=255)                                 ttcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGa                                                       <  1:1467883/49‑1 (MQ=255)                                  tcACGCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCa                                            >  1:2875631/1‑59 (MQ=255)                                      gCTGGTGGAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTAt                            <  1:571114/71‑1 (MQ=255)                                        tggtggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTAt                            <  1:1059094/69‑1 (MQ=255)                                        tggtggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATcg                          >  1:753146/1‑71 (MQ=255)                                           tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:478328/37‑1 (MQ=255)                                           tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:1385244/37‑1 (MQ=255)                                           tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:75285/37‑1 (MQ=255)                                           tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:1509326/37‑1 (MQ=255)                                           tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:586115/37‑1 (MQ=255)                                           tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:1618681/37‑1 (MQ=255)                                           tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:2101861/37‑1 (MQ=255)                                           tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:2483700/37‑1 (MQ=255)                                           tggAGGTGAATGTTGCCTGTGGATGTTTTGCGGTAtt                                                         <  1:217027/37‑1 (MQ=255)                                             gAGGTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTc                     <  1:2358414/71‑1 (MQ=255)                                               ggTGAATGTTGCCTGTGGATGTTTTGTGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGt                       >  1:732182/1‑67 (MQ=255)                                               ggTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCgg                   >  1:330041/1‑71 (MQ=255)                                               ggTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCgg                   >  1:2426326/1‑71 (MQ=255)                                               ggTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCgg                   >  1:654553/1‑71 (MQ=255)                                                gTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:1916521/42‑1 (MQ=255)                                                gTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:1471698/42‑1 (MQ=255)                                                gTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:1692471/42‑1 (MQ=255)                                                gTGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:1903800/42‑1 (MQ=255)                                                 tGAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCt                                               <  1:435099/41‑1 (MQ=255)                                                  gAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCAc                                           <  1:1910285/44‑1 (MQ=255)                                                  gAATGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCAc                                           <  1:2520282/44‑1 (MQ=255)                                                   aaTGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGt                <  1:851039/70‑1 (MQ=255)                                                    aTGTTGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGa              >  1:1481310/1‑71 (MQ=255)                                                       ttGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGt                       >  1:1088968/1‑59 (MQ=255)                                                       ttGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGt                       >  1:2499249/1‑59 (MQ=255)                                                       gtGCCTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGt                       >  1:2927676/2‑59 (MQ=255)                                                          ccTGTGGATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGATTAGt         <  1:1479781/70‑1 (MQ=255)                                                                gATGTTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGATTAGTATCGACg  >  1:864899/1‑71 (MQ=255)                                                                   |                                                                    CGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCACGCTGGTGGAGGTGAATGTTGCCTGTGGATATTTTGCGGTATTGAGGAAATCTGCACTGCGAAGATGTTTATCGCGTTCGGCGTGATTAGTATCGACG  >  minE/708935‑709068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑