Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	753,855	T→C	N189S (AAC→AGC)	ycfT ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	753,855	0	T	C	100.0%	175.9 / NA	48	N189S (AAC→AGC)	ycfT	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/30);  total (18/30)

AGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAAT  >  minE/753790‑753920                                                                  |                                                                  aGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCAtt                                                              >  1:2747419/1‑71 (MQ=255)   gTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCa                                                                >  1:2461497/1‑67 (MQ=255)   gTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTcc                                                            <  1:2469259/71‑1 (MQ=255)   gTCATTATTGTTGCGCCAAACCATGAGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCa                                                                >  1:884416/1‑67 (MQ=255)     cATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTccc                                                           <  1:32383/70‑1 (MQ=255)                cgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGt                                                <  1:140788/70‑1 (MQ=255)                cgcCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTg                                               <  1:659935/71‑1 (MQ=255)                  ccAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTggg                                             <  1:3125510/71‑1 (MQ=255)                  ccAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTggg                                             <  1:219108/71‑1 (MQ=255)                     aaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAAc                                          >  1:1472163/1‑71 (MQ=255)                     aaCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAAc                                          >  1:2221380/1‑71 (MQ=255)                      aCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAAc                                          >  1:1091532/1‑70 (MQ=255)                       ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGTATCACACTGCTCATTCCCCACCACGGCGTGGGAACg                                         <  1:2204749/70‑1 (MQ=255)                       ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTg                                               >  1:3055071/1‑64 (MQ=255)                       ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACg                                         <  1:1992672/70‑1 (MQ=255)                       ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACg                                         <  1:926536/70‑1 (MQ=255)                       ccATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGa                                        <  1:20248/71‑1 (MQ=255)                          tGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAt                                     <  1:1339763/71‑1 (MQ=255)                            cgcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt                                    <  1:66723/70‑1 (MQ=255)                            cgcCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt                                    <  1:742541/70‑1 (MQ=255)                              ccAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAAc                                 >  1:3174133/1‑71 (MQ=255)                              ccAAGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAAc                                 >  1:3082/1‑71 (MQ=255)                                 aGGCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGc                              <  1:1917549/71‑1 (MQ=255)                                   gCTGTAATAAGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCca                            >  1:1768077/1‑71 (MQ=255)                                       taataaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCcaca                          <  1:1697704/69‑1 (MQ=255)                                       taataaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACt                        >  1:2713768/1‑71 (MQ=255)                                       taataaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACt                        >  1:70420/1‑71 (MQ=255)                                          taaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag                     <  1:2554921/71‑1 (MQ=255)                                           aaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag                     >  1:1063048/1‑70 (MQ=255)                                           aaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag                     >  1:2277420/1‑70 (MQ=255)                                           aaGGCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCAACACTcag                     >  1:2527419/1‑70 (MQ=255)                                              gCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCg                                                 <  1:2775621/39‑1 (MQ=255)                                              gCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCg                                                 <  1:838986/39‑1 (MQ=255)                                              gCAAATTGCGGATCACACTGCTCATTCCCCACCACGGCg                                                 <  1:315378/39‑1 (MQ=255)                                                 aaTTGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTaca              <  1:1691203/71‑1 (MQ=255)                                                   ttGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGa                                        >  1:1537721/1‑43 (MQ=255)                                                   ttGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAt                                     <  1:3063682/46‑1 (MQ=255)                                                   ttGCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAt                                     <  1:3082322/46‑1 (MQ=255)                                                     gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt                                    <  1:3143541/45‑1 (MQ=255)                                                     gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt                                    <  1:1513547/45‑1 (MQ=255)                                                     gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt                                    <  1:2863109/45‑1 (MQ=255)                                                     gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAAtt                                    <  1:915344/45‑1 (MQ=255)                                                     gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTa                                   >  1:1430063/1‑46 (MQ=255)                                                     gCGGATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTa                                   >  1:766321/1‑46 (MQ=255)                                                       ggATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcagcag                  <  1:3021792/61‑1 (MQ=255)                                                       ggATCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcagcag                  <  1:2868103/61‑1 (MQ=255)                                                          tCACACTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCg     <  1:1837676/71‑1 (MQ=255)                                                             cacTGCTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAAt  <  1:991527/71‑1 (MQ=255)                                                                  |                                                                  AGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAAT  >  minE/753790‑753920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑