| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 780,667 | T→C | D125D (GAT→GAC) | ycgM → | predicted isomerase/hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 780,667 | 0 | T | C | 100.0% | 107.8 / NA | 30 | D125D (GAT→GAC) | ycgM | predicted isomerase/hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/18); total (12/18) | |||||||||||
ACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGC > minE/780599‑780734 | aCATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACaa > 1:944703/1‑71 (MQ=255) cGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCtt < 1:1181500/70‑1 (MQ=255) gTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGt < 1:2991026/71‑1 (MQ=255) aTGTTCGGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCg < 1:403824/71‑1 (MQ=255) cAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTc > 1:2724841/1‑71 (MQ=255) aGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTcc < 1:507943/71‑1 (MQ=255) aGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTcc < 1:2073279/71‑1 (MQ=255) gggAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTcc < 1:3123505/70‑1 (MQ=255) aaTGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTa > 1:783260/1‑71 (MQ=255) aTGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTAt < 1:2421310/71‑1 (MQ=255) gaagaaAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTc > 1:19960/1‑71 (MQ=255) gaagaaAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTc > 1:16157/1‑71 (MQ=255) acaaaGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGttt < 1:1097910/63‑1 (MQ=255) ccGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTa > 1:1117757/1‑60 (MQ=255) cGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCgcggc < 1:1474060/69‑1 (MQ=255) aGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAAtt > 1:2372100/1‑70 (MQ=255) ccGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCttt < 1:2263131/43‑1 (MQ=255) ccGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCttt < 1:1441736/43‑1 (MQ=255) ccGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCttt < 1:612583/43‑1 (MQ=255) ccGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTAc < 1:3070958/71‑1 (MQ=255) ccGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGGCCGCttt < 1:2849973/43‑1 (MQ=37) ccGTGGGAAAAGGCTAAAGCGTTT‑ACAACTCTTGTCCGCttt < 1:2854303/42‑1 (MQ=255) cGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTAcc < 1:2100290/71‑1 (MQ=255) cGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTAcc < 1:1562422/71‑1 (MQ=255) gTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCg < 1:203151/71‑1 (MQ=255) tGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCAGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCgg > 1:1639640/1‑71 (MQ=255) aaGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGAccc > 1:726579/1‑71 (MQ=255) tAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCaa > 1:1436652/1‑70 (MQ=255) cGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATAc > 1:2911961/1‑70 (MQ=255) tGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGc > 1:2795224/1‑71 (MQ=255) | ACATTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATACAACGC > minE/780599‑780734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |