| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 965,646 | C→T | G83D (GGC→GAC) | ydeM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 965,646 | 0 | C | T | 98.4% | 201.6 / ‑6.6 | 63 | G83D (GGC→GAC) | ydeM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (30/31); minor base G (0/1); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTT > minE/965581‑965709 | aGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCa > 1:1425139/1‑71 (MQ=255)aGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCa < 1:2991025/71‑1 (MQ=255) acacCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCa < 1:2803402/67‑1 (MQ=255) acacCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGc > 1:988054/1‑71 (MQ=255) acacCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGc > 1:2778223/1‑71 (MQ=255) cattcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttg > 1:109254/1‑71 (MQ=255) cattcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttg < 1:2141430/71‑1 (MQ=255) cattcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttg < 1:2787718/71‑1 (MQ=255) cattcattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttg < 1:1462442/71‑1 (MQ=255) cattATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGa > 1:217683/1‑71 (MQ=255) attattCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGa > 1:371719/1‑70 (MQ=255) attattCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGa < 1:2154191/70‑1 (MQ=255) attattCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGa < 1:1639876/70‑1 (MQ=255) attattCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGa > 1:33686/1‑70 (MQ=255) ttattCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATa < 1:762436/71‑1 (MQ=255) aaTAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTg < 1:805059/49‑1 (MQ=255) tAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttgtt < 1:1462324/59‑1 (MQ=255) tGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa > 1:514741/1‑71 (MQ=255) tGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa > 1:1009040/1‑71 (MQ=255) tGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa > 1:641241/1‑71 (MQ=255) ccATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTg < 1:1275989/40‑1 (MQ=255) ccATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTg < 1:2600468/40‑1 (MQ=255) ccATTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAA‑AACTTTACg > 1:2602804/1‑70 (MQ=255) aTTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCa > 1:2058378/1‑40 (MQ=255) aTTCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCa > 1:2785602/1‑40 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGc < 1:3157523/38‑1 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGc < 1:1142826/38‑1 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGc < 1:2635246/38‑1 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACgg < 1:421201/69‑1 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACgg < 1:3006700/69‑1 (MQ=255) ttCGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGa > 1:752651/1‑70 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaa < 1:3083230/70‑1 (MQ=255) cGTTTGTAATGCATTAAAAATACGTTTTTGGGCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaa < 1:1013749/70‑1 (MQ=255) gTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaa < 1:1167064/70‑1 (MQ=255) gTTTGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGaaaa < 1:427388/70‑1 (MQ=255) tttGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:2634035/62‑1 (MQ=255) tttGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:1600177/62‑1 (MQ=255) tttGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:2920846/62‑1 (MQ=255) tttGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa < 1:2530795/62‑1 (MQ=255) ttGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAAt > 1:129250/1‑71 (MQ=255) tGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttgt > 1:1106755/1‑42 (MQ=255) tGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttgt > 1:2952037/1‑42 (MQ=255) tGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttgt > 1:2445863/1‑42 (MQ=255) tGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCgttgt > 1:23332/1‑42 (MQ=255) tGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa > 1:2989655/1‑60 (MQ=255) tGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa > 1:1953885/1‑60 (MQ=255) tGTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTa > 1:1986945/1‑60 (MQ=255) gTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAAt < 1:1569409/69‑1 (MQ=255) gTAATGCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATc < 1:2290535/70‑1 (MQ=255) gCATTAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGgc > 1:2330693/1‑70 (MQ=255) ttAAAAATACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACgg > 1:564813/1‑54 (MQ=255) ttAAAAATACGTTTTTGG‑‑TGCATAGCGTTGTTGATAGTGAATAACTTTACgg > 1:3058785/1‑52 (MQ=38) aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTAc > 1:507379/1‑49 (MQ=255) aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTAc > 1:523969/1‑49 (MQ=255) aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGccagccag < 1:195655/71‑1 (MQ=255) aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGccagccag < 1:64140/71‑1 (MQ=255) aaaaTACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGccagccag < 1:2540185/71‑1 (MQ=255) aaaTACGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGccagccag < 1:1962380/70‑1 (MQ=255) aCGTTTTTGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTgg > 1:692696/1‑71 (MQ=255) tttttGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGtt > 1:958106/1‑71 (MQ=255) tttttGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGtt < 1:229389/71‑1 (MQ=255) ttttGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGtt > 1:2244026/1‑70 (MQ=255) ttGGTCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGt > 1:466753/1‑63 (MQ=255) | AGGCACACCATTCATTATTCAATAAAATGCCATTCGTTTGTAATGCATTAAAAATACGTTTTTGGCCTGCATAGCGTTGTTGATAGTGAATAACTTTACGGAAAAAATCCAGGCCAGCCAGAGTGGGTT > minE/965581‑965709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |