| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,019,797 | T→C | Y249C (TAT→TGT) | uidC ← | predicted outer membrane porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,019,797 | 0 | T | C | 98.2% | 194.0 / ‑4.1 | 54 | Y249C (TAT→TGT) | uidC | predicted outer membrane porin protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (26/27); minor base A (0/1); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTG > minE/1019732‑1019861 | ggctggTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTc > 1:2029175/1‑71 (MQ=255) ctggTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCgg < 1:2869812/71‑1 (MQ=255) ctggTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCgg < 1:31052/71‑1 (MQ=255) gTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTc > 1:2408132/1‑71 (MQ=255) tATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCg < 1:2669264/66‑1 (MQ=255) tATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCg < 1:2926871/71‑1 (MQ=255) ttACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGa < 1:387571/70‑1 (MQ=255) ttACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAg < 1:954909/71‑1 (MQ=255) tACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGc > 1:3008612/1‑71 (MQ=255) tACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAAATCCCCTTTCAACGTACAATTCGGGTCGAg < 1:241252/70‑1 (MQ=255) aCTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTa < 1:1401720/71‑1 (MQ=255) aCTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTa < 1:1973675/71‑1 (MQ=255) aaaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGc < 1:1772613/70‑1 (MQ=255) aaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTc > 1:1439132/1‑53 (MQ=255) aaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTc > 1:64808/1‑53 (MQ=255) aaCCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCtt < 1:797568/71‑1 (MQ=255) tCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTAt > 1:829995/1‑62 (MQ=255) tCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTtctc > 1:1256375/1‑71 (MQ=255) cGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTtctc < 1:2578387/70‑1 (MQ=255) aGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATg > 1:1552677/1‑60 (MQ=255) aGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCgag < 1:2745835/71‑1 (MQ=255) aGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGGCGAGCGGTATGCTTTTCTCgag < 1:1776202/71‑1 (MQ=255) cTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCgg < 1:970164/54‑1 (MQ=255) tGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCgg > 1:584379/1‑44 (MQ=255) gcgcGTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATa > 1:2190548/1‑71 (MQ=255) gcgcGTAAAAGCCGAGCAACTCCCCTTTCAACGTAAAATTCGGGTCGAGCGGTATGCTTTtct < 1:444231/63‑1 (MQ=255) gcgTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCg > 1:2788701/1‑63 (MQ=255) gcgTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACt < 1:440084/71‑1 (MQ=255) gTAAAAGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACttt < 1:1705031/71‑1 (MQ=255) aaaaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCg < 1:2936456/59‑1 (MQ=255) aaaaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTg > 1:1056461/1‑70 (MQ=255) aaaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTa > 1:2512209/1‑71 (MQ=255) aaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTtct > 1:2579200/1‑55 (MQ=255) aaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCgag > 1:65161/1‑59 (MQ=255) aaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCgag > 1:2039665/1‑59 (MQ=255) aaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTggga > 1:1951065/1‑68 (MQ=255) aaGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGGCGAGCGGTATGCTTTTCTCGAGAATACTTTGTAt < 1:1521175/71‑1 (MQ=255) aGCCGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATc > 1:2680499/1‑71 (MQ=255) cGAGCATCTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGt < 1:3105071/65‑1 (MQ=255) cGAGCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGt < 1:1763759/65‑1 (MQ=255) gCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGAtt < 1:390816/70‑1 (MQ=255) gCAACTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTg < 1:2381306/71‑1 (MQ=255) aaCTCCCCTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACttt < 1:2545138/58‑1 (MQ=255) cccTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAAc < 1:1409277/69‑1 (MQ=255) cccTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACAt < 1:1599414/71‑1 (MQ=255) cTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCt > 1:261125/1‑71 (MQ=255) cTTTCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCt > 1:1638439/1‑71 (MQ=255) ttCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACttt > 1:559986/1‑49 (MQ=255) ttCAACGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGaaa > 1:1216814/1‑64 (MQ=255) tCAACGTACAATTCGGGTCGAGCGGTATGCTTTtctc > 1:3138131/1‑37 (MQ=255) tCAACGTACAATTCGGGTCGAGCGGTATGCTTTtctc > 1:2629678/1‑37 (MQ=255) tCAACGTACAATTCGGGTCGAGCGGTATGCTTTtctc > 1:2064026/1‑37 (MQ=255) aCGTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTg > 1:2630642/1‑70 (MQ=255) gTACAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATc < 1:2803031/48‑1 (MQ=255) | GGCTGGTATTACGACTTAAACCTTCGAGCTGCGCGTAAAAGCCGAGCAACTCCCCTTTCAACGTATAATTCGGGTCGAGCGGTATGCTTTTCTCGAGAATACTTTGTATCTGATTGGAAACATCTTTCTG > minE/1019732‑1019861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |