Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,038,735 G→C V73L (GTG→CTG)  rsxE → predicted inner membrane NADH‑quinone reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,038,7350GC100.0% 414.6 / NA 112V73L (GTG→CTG) rsxEpredicted inner membrane NADH‑quinone reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (47/65);  total (47/65)

Only 100 of 112 total aligned reads displayed.

CGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTG  >  minE/1038667‑1038800
                                                                    |                                                                 
cGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtg                                                                 <  1:219693/71‑1 (MQ=255)
 gCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtga                                                                <  1:2532625/71‑1 (MQ=255)
 gCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtga                                                                >  1:1505494/1‑71 (MQ=255)
 gCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtga                                                                >  1:1501814/1‑71 (MQ=255)
    gACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgatga                                                             >  1:3113575/1‑71 (MQ=255)
    gACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCtgatga                                                             >  1:1488982/1‑71 (MQ=255)
     aCCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTgatgat                                                            >  1:14336/1‑71 (MQ=255)
      ccAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGatc                                                           <  1:2440152/71‑1 (MQ=255)
        aaCCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGatcat                                                         <  1:2224548/71‑1 (MQ=255)
        aaCCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGatcat                                                         <  1:3099005/71‑1 (MQ=255)
         aCCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGatcatc                                                        <  1:3048717/71‑1 (MQ=255)
         aCCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGatcatc                                                        <  1:1252528/71‑1 (MQ=255)
         aCCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGatcatc                                                        <  1:127517/71‑1 (MQ=255)
         aCCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGatcatc                                                        <  1:442593/71‑1 (MQ=255)
              aCCATTTCGACGCTGCGTCACTGGACTCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGcc                                                     <  1:2383175/69‑1 (MQ=255)
              aCCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGcc                                                     <  1:1761373/69‑1 (MQ=255)
              aCCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGcc                                                     <  1:3022622/69‑1 (MQ=255)
              aCCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGcc                                                     <  1:554389/69‑1 (MQ=255)
              aCCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGcc                                                     <  1:2954193/69‑1 (MQ=255)
                cATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg                                                 <  1:1963313/71‑1 (MQ=255)
                 aTTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggt                                                <  1:2414646/71‑1 (MQ=255)
                  tttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGatc                                                           <  1:2361988/59‑1 (MQ=255)
                   ttCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtgg                                              >  1:2970797/1‑71 (MQ=255)
                    tCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtggt                                             >  1:122006/1‑71 (MQ=255)
                    tCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtggt                                             >  1:192961/1‑71 (MQ=255)
                    tCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtggt                                             >  1:388810/1‑71 (MQ=255)
                    tCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtgg                                              <  1:738049/70‑1 (MQ=255)
                    tCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtgg                                              <  1:1927366/70‑1 (MQ=255)
                    tCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCggtgg                                              <  1:681430/70‑1 (MQ=255)
                    tCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg                                                 <  1:1674482/67‑1 (MQ=255)
                    tCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg                                                 <  1:1811968/67‑1 (MQ=255)
                    tCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCgg                                                 <  1:151838/67‑1 (MQ=255)
                      gACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTc                                            <  1:1655049/70‑1 (MQ=255)
                        cGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgc                                         >  1:1394414/1‑71 (MQ=255)
                         gCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGatca                                                          <  1:699162/53‑1 (MQ=255)
                         gCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgcg                                        <  1:92999/71‑1 (MQ=255)
                          cTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgcgc                                       <  1:528954/71‑1 (MQ=255)
                          cTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgcgc                                       <  1:1249936/71‑1 (MQ=255)
                          cTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgcgc                                       <  1:2152064/71‑1 (MQ=255)
                          cTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAgcg                                        <  1:1255153/70‑1 (MQ=255)
                             cGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTg                                     >  1:2793428/1‑70 (MQ=255)
                             cGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTg                                     >  1:1979901/1‑70 (MQ=255)
                             cGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTg                                     >  1:1423515/1‑70 (MQ=255)
                               tCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTAc                                  <  1:3049940/71‑1 (MQ=255)
                                   tGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGAt                              >  1:1603341/1‑71 (MQ=255)
                                   tGGACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGAt                              >  1:1388078/1‑71 (MQ=255)
                                    ggACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATg                             >  1:1323997/1‑71 (MQ=255)
                                    ggACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATg                             >  1:1546539/1‑71 (MQ=255)
                                     gACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGTTGGTCAGCGCTGTACAGATGc                            >  1:356597/1‑71 (MQ=255)
                                     gACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGAt                              <  1:1087993/69‑1 (MQ=255)
                                     gACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGAt                              <  1:1439895/69‑1 (MQ=255)
                                     gACGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGc                            >  1:759343/1‑71 (MQ=255)
                                      aCGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCt                           <  1:1597100/71‑1 (MQ=255)
                                       cGCCAGCCGAGATCCGCATTCCCATTTACCtgatg                                                              <  1:921491/35‑1 (MQ=255)
                                       cGCCAGCCGAGATCCGCATTCCCATTTACCtgatg                                                              <  1:1992021/35‑1 (MQ=255)
                                       cGCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCt                           >  1:2271181/1‑70 (MQ=255)
                                        gCCAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGa                         <  1:2780820/71‑1 (MQ=255)
                                         ccAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt                        <  1:7030/71‑1 (MQ=255)
                                          cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAg                                          <  1:496585/52‑1 (MQ=255)
                                          cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAg                                          <  1:2647271/52‑1 (MQ=255)
                                          cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAg                                          <  1:2650781/52‑1 (MQ=255)
                                          cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAg                                          <  1:2001864/52‑1 (MQ=255)
                                          cAGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAg                                          <  1:765871/52‑1 (MQ=255)
                                           aGCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCa                      <  1:237628/71‑1 (MQ=255)
                                            gCCGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCaa                     >  1:1047869/1‑71 (MQ=255)
                                             ccGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    <  1:777345/71‑1 (MQ=255)
                                             ccGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    <  1:2381135/71‑1 (MQ=255)
                                             ccGAGATCCGCATTCCCATTAACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    >  1:708925/1‑71 (MQ=255)
                                              cGAGATCCTCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAac                    <  1:886536/70‑1 (MQ=255)
                                              cGAGATCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacg                   <  1:186746/71‑1 (MQ=255)
                                                agaTCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGc                            >  1:1207853/1‑60 (MQ=255)
                                                agaTCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGc                            >  1:1614521/1‑60 (MQ=255)
                                                  aTCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgccta               >  1:2319612/1‑71 (MQ=255)
                                                   tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcctac              <  1:717439/71‑1 (MQ=255)
                                                   tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcctac              <  1:546404/71‑1 (MQ=255)
                                                   tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcctac              <  1:1651037/71‑1 (MQ=255)
                                                   tCCGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcctac              <  1:2049915/71‑1 (MQ=255)
                                                    ccGCATTCCCATTTACCTGATGATCATCGCCTCgg                                                 >  1:2814693/1‑35 (MQ=255)
                                                    ccGCATTCCCATTTACCTGATGATCATCGCCTCgg                                                 >  1:662540/1‑35 (MQ=255)
                                                    ccGCATTCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacgcctacg             >  1:2912013/1‑71 (MQ=255)
                                                         ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGa                               >  1:3069737/1‑48 (MQ=255)
                                                         ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGAt                        >  1:1819071/1‑55 (MQ=255)
                                                         ttCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCttt        >  1:371262/1‑71 (MQ=255)
                                                          tCCCATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTg       >  1:2759832/1‑71 (MQ=255)
                                                           cccATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCaa                     >  1:1558520/1‑56 (MQ=255)
                                                             cATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTg                                     <  1:678008/38‑1 (MQ=255)
                                                             cATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGcc    <  1:1578081/71‑1 (MQ=255)
                                                             cATTTACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGcc    <  1:720567/71‑1 (MQ=255)
                                                               tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAg                                >  1:532603/1‑41 (MQ=255)
                                                               tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacg                   <  1:1721090/54‑1 (MQ=255)
                                                               tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacg                   <  1:932393/54‑1 (MQ=255)
                                                               tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTg  >  1:2165570/1‑71 (MQ=255)
                                                               tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTg  <  1:1617689/71‑1 (MQ=255)
                                                               tttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTg  >  1:1223611/1‑71 (MQ=255)
                                                                ttACCTGATGATCATCGCCTCGGTGGTCAGCGCTg                                     <  1:1351314/35‑1 (MQ=255)
                                                                ttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacg                   >  1:1834165/1‑53 (MQ=255)
                                                                ttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacg                   >  1:2686951/1‑53 (MQ=255)
                                                                ttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacg                   >  1:1352511/1‑53 (MQ=255)
                                                                ttACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAacg                   >  1:17065/1‑53 (MQ=255)
                                                                 tACCTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTgg      >  1:1795374/1‑65 (MQ=255)
                                                                    |                                                                 
CGCTGACCAACCTGACCATTTCGACGCTGCGTCACTGGACGCCAGCCGAGATCCGCATTCCCATTTACGTGATGATCATCGCCTCGGTGGTCAGCGCTGTACAGATGCTGATCAACGCCTACGCCTTTGGCCTG  >  minE/1038667‑1038800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑