| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,091,167 | T→C | E178E (GAA→GAG) | sufB ← | component of SufBCD complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,091,167 | 0 | T | C | 97.2% | 127.3 / ‑3.0 | 36 | coding (534/1488 nt) | sufB | component of SufBCD complex |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (15/20); minor base . (1/0); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGC > minE/1091100‑1091234 | ccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTcc < 1:2480419/71‑1 (MQ=255) cgGCAAAGAAGTTGTCATT‑CCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTg < 1:2066330/69‑1 (MQ=255) gCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGAt < 1:2688863/71‑1 (MQ=255) agTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCa > 1:722758/1‑71 (MQ=255) gTTGTCATTCCCCGGCACCACGGTGCCGAGGTATTTACGCACCAGCTCCGGGTGATCGt > 1:366913/1‑59 (MQ=255) gTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGt > 1:801265/1‑59 (MQ=255) gTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTgg < 1:2941519/61‑1 (MQ=255) gTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCAc < 1:2542999/71‑1 (MQ=255) gTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCa > 1:1643969/1‑70 (MQ=255) gTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCa > 1:1647526/1‑70 (MQ=255) cATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAG‑‑CTGGGTGATCGTGGATc > 1:929244/1‑57 (MQ=255) aTTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAgg < 1:740279/71‑1 (MQ=255) ttCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGa < 1:1112898/71‑1 (MQ=255) ttCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGa < 1:551904/71‑1 (MQ=255) ccccGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGaa > 1:275844/1‑70 (MQ=255) ccccGGCACCACGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGaa > 1:1896878/1‑70 (MQ=255) cGGCACCACGGTGCCGAGATATTTACGCACCAGCGCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAg > 1:2682070/1‑70 (MQ=255) caccacGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGaaaa < 1:1584480/71‑1 (MQ=255) ccacGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaata < 1:2398778/71‑1 (MQ=255) cacGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCa < 1:649791/55‑1 (MQ=255) cacGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCa < 1:1628929/55‑1 (MQ=255) cGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGAt > 1:1830218/1‑43 (MQ=255) cGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATc < 1:534803/44‑1 (MQ=255) cGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaataat > 1:3010105/1‑70 (MQ=255) cGGTGCCGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAAtt < 1:17789/71‑1 (MQ=255) ccGAGATATTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTg > 1:1076889/1‑71 (MQ=255) atTTACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGc > 1:799574/1‑70 (MQ=255) tttACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaat < 1:1815033/54‑1 (MQ=255) ttACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAg > 1:2984787/1‑71 (MQ=255) ttACGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAg > 1:731722/1‑71 (MQ=255) cGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGttt < 1:1443742/71‑1 (MQ=255) cGCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGttt < 1:1184032/71‑1 (MQ=255) gCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCg < 1:2623944/63‑1 (MQ=255) gCACCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCg < 1:130236/63‑1 (MQ=255) aCCAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTc > 1:2799985/1‑71 (MQ=255) cAGCTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcgc < 1:1397656/71‑1 (MQ=255) | CCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGC > minE/1091100‑1091234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |