Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | minE | 1,093,658 | T→C | T907A (ACA→GCA) | ydiJ ← | predicted FAD‑linked oxidoreductase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,093,658 | 0 | T | C | 96.5% | 299.3 / 0.5 | 87 | T907A (ACA→GCA) | ydiJ | predicted FAD‑linked oxidoreductase |
Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (26/58); total (29/58) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.45e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 |
TGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCA > minE/1093590‑1093725 | tGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAg > 1:182367/1‑71 (MQ=255) tGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAg > 1:1783812/1‑71 (MQ=255) tGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTAACCTCTGACTGCAg > 1:136198/1‑71 (MQ=255) ggCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGc > 1:1591525/1‑71 (MQ=255) ggCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGc > 1:1466630/1‑71 (MQ=255) gcgcACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGcc < 1:1150731/71‑1 (MQ=255) gcgcACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGcc < 1:2703159/71‑1 (MQ=255) gcgcACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGcc < 1:2105639/71‑1 (MQ=255) gcACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCAc < 1:2698976/71‑1 (MQ=255) aCCCGGCAAGGCTGTAACTTCGGTACAGTGACCAAAGAAATACCATGTTTCACCGCTGACTGCAGCCACTg < 1:991656/71‑1 (MQ=255) cccGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTgg < 1:3103831/71‑1 (MQ=255) ccGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGc > 1:1568408/1‑71 (MQ=255) ccGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGc > 1:1628573/1‑71 (MQ=255) cGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCt < 1:2066898/71‑1 (MQ=255) cGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCt < 1:488000/71‑1 (MQ=255) ggCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCtg > 1:988358/1‑71 (MQ=255) ggCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCtg > 1:1481117/1‑71 (MQ=255) ggCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCtg > 1:2564117/1‑71 (MQ=255) gCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCtgt < 1:286102/71‑1 (MQ=255) aaGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGa < 1:3044913/71‑1 (MQ=255) ggTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCaa < 1:939279/71‑1 (MQ=255) gTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAg < 1:1456616/71‑1 (MQ=255) gTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAg < 1:2498688/71‑1 (MQ=255) gTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAg < 1:2937534/71‑1 (MQ=255) tAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCt < 1:1853493/60‑1 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGc < 1:2245474/46‑1 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGc < 1:244599/46‑1 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGc < 1:144112/46‑1 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCtt > 1:97096/1‑71 (MQ=255) cGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCt < 1:1270677/70‑1 (MQ=255) ggTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCtt < 1:970128/70‑1 (MQ=255) gTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCtt < 1:217078/69‑1 (MQ=255) aCAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTgc > 1:2029921/1‑69 (MQ=255) cAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCt < 1:2165286/49‑1 (MQ=255) cAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTgccag < 1:806010/71‑1 (MQ=255) cAGTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTgccag < 1:771433/71‑1 (MQ=255) gTGACCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTgccagc > 1:2292895/1‑70 (MQ=255) aCCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcatt > 1:2619076/1‑71 (MQ=255) aCCAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcatt > 1:1522010/1‑71 (MQ=255) cAAAGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTgcg < 1:334708/56‑1 (MQ=255) aaaGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCTAGCcattcat < 1:2333060/71‑1 (MQ=255) aaaGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattcat < 1:422657/71‑1 (MQ=255) aaaGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattcat < 1:1377632/71‑1 (MQ=255) aaGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattcatt > 1:1564606/1‑71 (MQ=255) aaGAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattcat < 1:1823719/70‑1 (MQ=255) gAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcatt < 1:1456727/65‑1 (MQ=255) gAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcatt < 1:2095769/65‑1 (MQ=255) gAAATACCATGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcatt < 1:497029/65‑1 (MQ=255) aTGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCattcattc < 1:352443/62‑1 (MQ=255) aTGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCattcattc < 1:1905653/62‑1 (MQ=255) aTGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCattcattc < 1:2507543/62‑1 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccagc > 1:1517794/1‑52 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccagc > 1:854721/1‑52 (MQ=255) tGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTgccagc > 1:185974/1‑52 (MQ=255) tGATTCACCGCTGACTGCAGCCACTGGCTGTGACt > 1:258734/1‑35 (MQ=255) tGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGa < 1:1516698/71‑1 (MQ=255) tGATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGa < 1:2117801/71‑1 (MQ=255) gATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattca < 1:1878989/57‑1 (MQ=255) gATTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattca < 1:2772101/57‑1 (MQ=255) aTTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattc < 1:3003694/55‑1 (MQ=255) aTTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattc < 1:3192884/55‑1 (MQ=255) aTTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattc < 1:2662503/55‑1 (MQ=255) aTTCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCcattc < 1:1991843/55‑1 (MQ=255) ttCACCGCTGACTGCAGCCTCTGGCTGTGACTCAAg > 1:2351158/1‑36 (MQ=37) ttCACCGCTGACTGCAGCCACTGGCTGTGACTCAAg > 1:886005/1‑36 (MQ=255) ttCACCGCTGACTGCAGCCACTGGCTGTGACTCAAg > 1:1892006/1‑36 (MQ=255) ttCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTgccggc < 1:848671/49‑1 (MQ=255) ttCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTgccagc < 1:973571/49‑1 (MQ=255) ttCACCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTgccagc < 1:1055923/49‑1 (MQ=255) aCCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTa < 1:1818549/70‑1 (MQ=255) aCCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTa < 1:2898612/70‑1 (MQ=255) aCCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTa > 1:249723/1‑70 (MQ=255) aCCGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTa < 1:1446516/70‑1 (MQ=255) ccGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCtt < 1:1405713/39‑1 (MQ=255) ccGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCtt < 1:1456601/39‑1 (MQ=255) ccGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGc > 1:646386/1‑56 (MQ=255) ccGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGc > 1:1304889/1‑56 (MQ=255) ccGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGc > 1:1234388/1‑56 (MQ=255) ccGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGc > 1:2524926/1‑56 (MQ=255) ccGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTaaa < 1:2340064/71‑1 (MQ=255) cGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTa < 1:1933032/68‑1 (MQ=255) cGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTa < 1:2059478/68‑1 (MQ=255) cGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTa < 1:1890404/68‑1 (MQ=255) cGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAAc > 1:705980/1‑71 (MQ=255) cGCTGACTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAAc < 1:322215/71‑1 (MQ=255) cTGCAGCCTCTGGCTGTGACTCAAGTGCGCTTGCCAGCcattca < 1:559358/44‑1 (MQ=38) cTGCAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCa < 1:1851526/71‑1 (MQ=255) | TGGCGCACCCGGCAAGGCGGTAACTTCGGTACAGTGACCAAAGAAATACCATGATTCACCGCTGACTGTAGCCACTGGCTGTGACTCAAGTGCGCTTGCCAGCCATTCATTCGCCAGTAAGACGTTAAACTCGCCA > minE/1093590‑1093725 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |