| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,216,702 | T→C | Q677Q (CAA→CAG) | prc ← | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,216,702 | 0 | T | C | 91.7% | 69.7 / ‑0.5 | 24 | Q677Q (CAA→CAG) | prc | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (13/9); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATC > minE/1216634‑1216764 | aTCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGgctg < 1:1476558/71‑1 (MQ=255)aTCTTGTCGCTGCTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGgctg < 1:1352208/71‑1 (MQ=255) tCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGgctgt > 1:1864299/1‑71 (MQ=255) gTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGCTGTTc < 1:248973/69‑1 (MQ=255) ttAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGt < 1:345761/69‑1 (MQ=255) aaaaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTg > 1:455365/1‑70 (MQ=255) aaaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGgctg < 1:2893112/56‑1 (MQ=255) aaaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGc > 1:1264021/1‑71 (MQ=255) aaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCttt > 1:1197505/1‑71 (MQ=255) acaATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCt < 1:3136076/71‑1 (MQ=255) aaTTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTc < 1:2574357/71‑1 (MQ=255) tttCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGc < 1:1886101/71‑1 (MQ=255) tttCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGc > 1:486027/1‑71 (MQ=255) ccTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAg < 1:147440/71‑1 (MQ=255) cTGATTGATATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGt < 1:449336/71‑1 (MQ=255) gattgatATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAg > 1:352378/1‑53 (MQ=255) attgatATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGc < 1:3062998/70‑1 (MQ=255) attgatATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTAGCTTTTTCAAGCTTCGCCAGATCGAGTGc < 1:2696673/70‑1 (MQ=255) attgatATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGc > 1:614443/1‑70 (MQ=255) attgatATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGc > 1:290489/1‑70 (MQ=255) tgatATTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAg > 1:1046303/1‑65 (MQ=255) atatTACTTGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGata < 1:1995158/70‑1 (MQ=255) ttcaCGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCAcc > 1:1767789/4‑69 (MQ=255) ttGACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCt < 1:2460298/43‑1 (MQ=255) gACGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGtct > 1:874443/1‑71 (MQ=255) aCGGGAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGata > 1:2613173/1‑60 (MQ=255) gggAGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCAt > 1:55188/1‑71 (MQ=255) aGCGGGCTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATc > 1:1401911/1‑69 (MQ=255) | ATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATC > minE/1216634‑1216764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |