Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,246,787	C→T	G70G (GGC→GGT)	znuC →	zinc transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,246,787	0	C	T	100.0%	282.5 / NA	85	G70G (GGC→GGT)	znuC	zinc transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/42);  total (43/42)

CGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTAC  >  minE/1246719‑1246854                                                                     |                                                                    cGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTa                                                                   >  1:2424009/1‑71 (MQ=255)  gggTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTAt                                                                  <  1:1378392/71‑1 (MQ=255)  gggTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTAt                                                                  <  1:1374845/71‑1 (MQ=255)     tAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTa                                                               <  1:528213/71‑1 (MQ=255)     tAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTa                                                               <  1:1855774/71‑1 (MQ=255)          cTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCg                                                            >  1:2501021/1‑69 (MQ=255)          cTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCg                                                            >  1:926273/1‑69 (MQ=255)           tCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCg                                                            >  1:140643/1‑68 (MQ=255)             gggCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAg                                                         >  1:1214100/1‑69 (MQ=255)             gggCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAg                                                         >  1:1942285/1‑69 (MQ=255)                 tGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTAc                                                              >  1:1734575/1‑60 (MQ=255)                  ggTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTg                                                   >  1:2597826/1‑70 (MQ=255)                  ggTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTg                                                   >  1:1270701/1‑70 (MQ=255)                   gTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGt                                                  <  1:2240777/70‑1 (MQ=255)                    tAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTAt                                                <  1:3029050/71‑1 (MQ=255)                     aaCACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGc                                                     <  1:1392063/65‑1 (MQ=255)                     aaCACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGc                                                     <  1:717836/65‑1 (MQ=255)                     aaCACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGc                                                     <  1:1509345/65‑1 (MQ=255)                     aaCACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTAtc                                               >  1:2565959/1‑71 (MQ=255)                      acacCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTAtct                                              >  1:2163646/1‑71 (MQ=255)                         cccGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGa                                           >  1:3016092/1‑71 (MQ=255)                          ccGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGa                                           >  1:746946/1‑70 (MQ=255)                              tGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCg                                                            >  1:2070372/1‑49 (MQ=255)                              tGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTAt                                                >  1:642997/1‑61 (MQ=255)                                  ggggTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGtt                                  <  1:487651/71‑1 (MQ=255)                                   gggTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCa                                                          <  1:2406711/46‑1 (MQ=255)                                     gTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGa                                                        >  1:1292709/1‑46 (MQ=255)                                     gTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGa                                                        >  1:818521/1‑46 (MQ=255)                                     gTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACAGCAGAATCTGTATCTCGACACCACGt                                   <  1:2215708/67‑1 (MQ=255)                                        aTCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACt                            >  1:938437/1‑71 (MQ=255)                                         tCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACt                            >  1:1504470/1‑70 (MQ=255)                                          cAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTTTATCTCGACACCACGtt                                  <  1:2201975/63‑1 (MQ=255)                                          cAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGtt                                  <  1:752142/63‑1 (MQ=255)                                          cAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGtt                                  <  1:587798/63‑1 (MQ=255)                                          cAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGtt                                  <  1:2922808/63‑1 (MQ=255)                                           aaGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGt                                                  >  1:2628177/1‑46 (MQ=255)                                             gcgcAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGAcc                        >  1:1164559/1‑70 (MQ=255)                                             gcgcAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCg                       >  1:2859905/1‑71 (MQ=255)                                             gcgcAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCg                       >  1:2441979/1‑71 (MQ=255)                                               gcAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGt                      >  1:2580813/1‑70 (MQ=255)                                               gcAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTa                     <  1:2565175/71‑1 (MQ=255)                                                cAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGt                                                  <  1:293/41‑1 (MQ=255)                                                cAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGt                                                  <  1:1402544/41‑1 (MQ=255)                                                 aaCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCt                                                    <  1:1228395/38‑1 (MQ=255)                                                 aaCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCt                                                    <  1:222082/38‑1 (MQ=255)                                                 aaCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCt                                                    <  1:1129147/38‑1 (MQ=255)                                                 aaCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGt                                                  <  1:1018705/40‑1 (MQ=255)                                                  aCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACg                                    >  1:2036528/1‑53 (MQ=255)                                                  aCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGt                                   >  1:2766347/1‑54 (MQ=255)                                                  aCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGc                                <  1:2752722/57‑1 (MQ=255)                                                  aCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGc                                <  1:1447420/57‑1 (MQ=255)                                                  aCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGa                          >  1:427453/1‑63 (MQ=255)                                                  aCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGa                          >  1:2562163/1‑63 (MQ=255)                                                  aCGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGa                          >  1:1289209/1‑63 (MQ=255)                                                   cGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAAcc                 >  1:1591072/1‑71 (MQ=255)                                                    ggAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCg                <  1:2079089/71‑1 (MQ=255)                                                    ggAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCg                <  1:887380/71‑1 (MQ=255)                                                     gAAAACTGCGGATCGGTTATGTACAGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGt               <  1:2699120/71‑1 (MQ=255)                                                     gAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGAcacca                                      >  1:20137/1‑48 (MQ=255)                                                     gAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGt               <  1:605452/71‑1 (MQ=255)                                                     gAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGt               <  1:1250856/71‑1 (MQ=255)                                                     gAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGt               >  1:953154/1‑71 (MQ=255)                                                      aaaaCTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGt               <  1:252397/70‑1 (MQ=255)                                                        aaCTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGtttt            <  1:1144181/71‑1 (MQ=255)                                                         aCTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGttttt           <  1:60424/71‑1 (MQ=255)                                                         aCTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGttttt           <  1:2084052/71‑1 (MQ=255)                                                          cTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAAcc                 >  1:2785682/1‑64 (MQ=255)                                                          cTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAAcc                 >  1:212879/1‑64 (MQ=255)                                                          cTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAAcc                 >  1:1665322/1‑64 (MQ=255)                                                           tGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGTCACCACGTTGCCACTGACCGTAAACCGt               <  1:2229135/65‑1 (MQ=255)                                                           tGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGt               <  1:1206111/65‑1 (MQ=255)                                                             cgcATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttac        <  1:1242635/70‑1 (MQ=255)                                                             cgcATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttac        <  1:1125700/70‑1 (MQ=255)                                                              gcATCGGTTATGTTCCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgc      <  1:1477683/71‑1 (MQ=255)                                                              gcATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgc      <  1:2820677/71‑1 (MQ=255)                                                              gcATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgc      <  1:2365343/71‑1 (MQ=255)                                                              gcATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgc      <  1:1086999/71‑1 (MQ=255)                                                                aTCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct     >  1:1441574/1‑70 (MQ=255)                                                                aTCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct     >  1:125385/1‑70 (MQ=255)                                                                aTCGGTTATGTACCGCAGAAGCTGTATCTAGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct     >  1:2837960/1‑70 (MQ=255)                                                                  cGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgcttac  >  1:46465/1‑71 (MQ=255)                                                                  cGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgcttac  >  1:3013208/1‑71 (MQ=255)                                                                  cGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTtta         >  1:450531/1‑64 (MQ=255)                                                                  cGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTtta         >  1:191208/1‑64 (MQ=255)                                                                  cGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTtta         >  1:1238405/1‑64 (MQ=255)                                                                     |                                                                    CGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTAC  >  minE/1246719‑1246854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑