BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,427,154	C→T	R220H (CGC→CAC)	napG ←	ferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,427,154	0	C	T	100.0%	347.1 / NA	103	R220H (CGC→CAC)	napG	ferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB)
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (55/48); total (55/48)

Only 100 of 103 total aligned reads displayed.

TTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAG  >  minE/1427086‑1427221

tttCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTgg                                                                   >  1:2022523/1‑71 (MQ=255)

  tctcCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGt                                                                  <  1:745963/70‑1 (MQ=255)

  tctcCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGt                                                                  <  1:1895582/70‑1 (MQ=255)

         cgcCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTg                                                           <  1:1104416/70‑1 (MQ=255)

                cgcCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGAc                                                         >  1:910982/1‑65 (MQ=255)

                cgcCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGAc                                                         >  1:663271/1‑65 (MQ=255)

                  cccGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTa                                                                 <  1:839707/55‑1 (MQ=255)

                   ccGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTccc                                                >  1:514357/1‑71 (MQ=255)

                     ggCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTc                                                  >  1:1776543/1‑67 (MQ=255)

                     ggCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCt                                              >  1:1041404/1‑71 (MQ=255)

                     ggCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCt                                              <  1:3129309/71‑1 (MQ=255)

                     ggCGTCACGTTTACGATTTGCCATTGTTACCCTCCAGCCAGCCGAAGTGGTAATgg                                                             >  1:1358519/1‑56 (MQ=255)

                      gCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCtt                                             <  1:1641007/71‑1 (MQ=255)

                       cGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCttt                                            >  1:1873190/1‑71 (MQ=255)

                        gTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTc                                           <  1:1341091/71‑1 (MQ=255)

                         tCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCtt                                             <  1:1255353/68‑1 (MQ=255)

                         tCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCtt                                             <  1:1557891/68‑1 (MQ=255)

                           aCGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTaa                                                                >  1:855305/1‑47 (MQ=255)

                           aCGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTaa                                                                >  1:1053458/1‑47 (MQ=255)

                           aCGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTaa                                                                >  1:2626038/1‑47 (MQ=255)

                           aCGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTaa                                                                >  1:724216/1‑47 (MQ=255)

                           aCGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTg                                                           >  1:780210/1‑52 (MQ=255)

                           aCGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGcc                                        <  1:1991744/71‑1 (MQ=255)

                           aCGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGcc                                        <  1:1672079/71‑1 (MQ=255)

                              tttACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGt                                     >  1:1482834/1‑71 (MQ=255)

                              tttACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGt                                     >  1:422108/1‑71 (MQ=255)

                               ttACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCtt                                             <  1:1783346/62‑1 (MQ=255)

                               ttACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGt                                     >  1:39861/1‑70 (MQ=255)

                               ttACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGt                                     >  1:3060704/1‑70 (MQ=255)

                               ttACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGt                                     >  1:1197406/1‑70 (MQ=255)

                               ttACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGt                                     >  1:228566/1‑70 (MQ=255)

                               ttACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGt                                     >  1:1649685/1‑70 (MQ=255)

                                tACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTg                                    <  1:16002/70‑1 (MQ=255)

                                tACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGa                                   >  1:2332533/1‑71 (MQ=255)

                                tACGATTTGCCATTGTTCCCCTCCAGCCAGCAGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTg                                    <  1:1522108/70‑1 (MQ=255)

                                 aCGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGTTAATGGTGACCTAACTCCCCTTTCGCCa                                       <  1:2309723/66‑1 (MQ=255)

                                 aCGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCa                                       <  1:2017149/66‑1 (MQ=255)

                                 aCGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGa                                   >  1:240633/1‑70 (MQ=255)

                                 aCGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGa                                   >  1:1808557/1‑70 (MQ=255)

                                  cGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGAc                                  <  1:2196851/70‑1 (MQ=255)

                                     tttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGcc                                        <  1:2280104/61‑1 (MQ=255)

                                     tttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGcc                                        <  1:2770280/61‑1 (MQ=255)

                                     tttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGcc                                        <  1:2371289/61‑1 (MQ=255)

                                     tttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCg                              >  1:1125398/1‑71 (MQ=255)

                                     tttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCg                              <  1:2237979/71‑1 (MQ=255)

                                     tttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCg                              <  1:703332/71‑1 (MQ=255)

                                     tttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCg                              <  1:1072325/71‑1 (MQ=255)

                                      ttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCgg                             >  1:621183/1‑71 (MQ=255)

                                      ttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCgg                             >  1:2210674/1‑71 (MQ=255)

                                      ttGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCg                              <  1:1828852/70‑1 (MQ=255)

                                       tGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAg                                      >  1:1201808/1‑61 (MQ=255)

                                       tGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAg                                      >  1:209382/1‑61 (MQ=255)

                                       tGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAg                                      >  1:141830/1‑61 (MQ=255)

                                       tGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCg                              <  1:909085/69‑1 (MQ=255)

                                       tGCCATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCg                              <  1:1010426/69‑1 (MQ=255)

                                         ccATTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTaa                          >  1:3154849/1‑71 (MQ=255)

                                           aTTGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTcc                                                 <  1:1234612/46‑1 (MQ=255)

                                            ttGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAg                                      <  1:2508799/56‑1 (MQ=255)

                                            ttGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAg                                      <  1:517298/56‑1 (MQ=255)

                                            ttGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAacac                       >  1:493657/1‑71 (MQ=255)

                                            ttGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAacac                       >  1:1584235/1‑71 (MQ=255)

                                            atGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAacac                       >  1:2778510/2‑71 (MQ=255)

                                             tGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACAcc                      >  1:894573/1‑71 (MQ=255)

                                             tGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACAcc                      >  1:8905/1‑71 (MQ=255)

                                             tGTTCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACAcc                      >  1:72237/1‑71 (MQ=255)

                                               ttCCCCTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCtt                    <  1:1343196/71‑1 (MQ=255)

                                                 ccccTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGa                  >  1:487622/1‑71 (MQ=255)

                                                  cccTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCttt                                            <  1:205020/44‑1 (MQ=255)

                                                  cccTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCttt                                            <  1:2674443/44‑1 (MQ=255)

                                                  cccTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCttt                                            <  1:1966696/44‑1 (MQ=255)

                                                  cccTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGAt                 >  1:198603/1‑71 (MQ=255)

                                                   ccTCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGAtt                >  1:2661419/1‑71 (MQ=255)

                                                     tCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGAc                                  <  1:1262507/51‑1 (MQ=255)

                                                     tCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAg                                >  1:3018815/1‑53 (MQ=255)

                                                     tCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAg                                >  1:649647/1‑53 (MQ=255)

                                                     tCCAGCCAGCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAg                                >  1:2341246/1‑53 (MQ=255)

                                                      ccagccagCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGcc             >  1:1706565/1‑71 (MQ=255)

                                                      ccagccagCCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGcc             >  1:1131208/1‑71 (MQ=255)

                                                       cagccagcCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGcc             <  1:443920/70‑1 (MQ=255)

                                                       cagccagcCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGcc             <  1:2814939/70‑1 (MQ=255)

                                                       cagccagcCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGcc             <  1:320554/70‑1 (MQ=255)

                                                       cagccagcCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGcc             <  1:2999747/70‑1 (MQ=255)

                                                       cagccagcCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGc              <  1:676046/69‑1 (MQ=255)

                                                       cagccagcCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCg            >  1:1878469/1‑71 (MQ=255)

                                                       cagccagcCGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACATTGATTGCCg            >  1:2391837/1‑71 (MQ=255)

                                                       aagccagccGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCg            >  1:749452/2‑71 (MQ=255)

                                                           cagccGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACAcc                      <  1:2214906/57‑1 (MQ=255)

                                                           cagccGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGgttg        <  1:1176890/71‑1 (MQ=255)

                                                            agccGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGgttgt       <  1:2470424/71‑1 (MQ=255)

                                                             gccGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGgttgtt      <  1:793856/71‑1 (MQ=255)

                                                               cGAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTcc    <  1:569716/71‑1 (MQ=255)

                                                                gAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTc     >  1:1715284/1‑69 (MQ=255)

                                                                gAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTc     >  1:1612046/1‑69 (MQ=255)

                                                                gAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTc     >  1:1474898/1‑69 (MQ=255)

                                                                gAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTc     >  1:2208232/1‑69 (MQ=255)

                                                                gAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTc     >  1:1985149/1‑69 (MQ=255)

                                                                gAAGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCa   <  1:37762/71‑1 (MQ=255)

                                                                 aaGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAg  >  1:3182411/1‑71 (MQ=255)

                                                                 aaGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAg  <  1:1672401/71‑1 (MQ=255)

                                                                 aaGTGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAg  >  1:2737123/1‑71 (MQ=255)

TTTCTCCAGCGCCTCGCGCCCGGCGTCACGTTTACGATTTGCCATTGTTCCCCTCCAGCCAGCCGAAGCGGTAATGGTGACCTAACTCCCCTTTCGCCAGTGACAGCGGTAACACCTTGATTGCCGGTTGTTCCAG  >  minE/1427086‑1427221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`