Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	21,090	A→G	D341G (GAC→GGC)	ileS →	isoleucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	21,090	0	A	G	100.0%	98.3 / NA	28	D341G (GAC→GGC)	ileS	isoleucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/17);  total (11/17)

CTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACT  >  minE/21023‑21154                                                                    |                                                                 cTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGggcga                                                               >  1:3035948/1‑71 (MQ=255) cTCGGCGATCACGTTACCCTGGATGCAGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGggcga                                                               >  1:253303/1‑71 (MQ=255)  tCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGggcgac                                                              >  1:83090/1‑71 (MQ=255)  tCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGggcgac                                                              >  1:1094483/1‑71 (MQ=255)        aTCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACt                                                             <  1:366787/66‑1 (MQ=255)        aTCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACt                                                             <  1:2267111/66‑1 (MQ=255)         tCAAGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGa                                                       >  1:87756/1‑71 (MQ=255)             gTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCTCGCCTGGCCACGGCCCGGGCGACTATGTGATCgg                                                   >  1:1142236/1‑71 (MQ=255)                    tGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGaaa                                            >  1:299033/1‑71 (MQ=255)                    tGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATATGATCGGTCAGaaa                                            >  1:3022340/1‑71 (MQ=255)                      gATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTa                                                            >  1:1468330/1‑53 (MQ=255)                      gATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAAt                                           <  1:1823825/70‑1 (MQ=255)                           cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGcc                                     <  1:1209011/71‑1 (MQ=255)                             gTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTg                                   >  1:145092/1‑71 (MQ=255)                                ccGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGa                                 <  1:2695053/70‑1 (MQ=255)                                  ggTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAAc                              <  1:2357951/71‑1 (MQ=255)                                   gTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGcc                                     >  1:2995727/1‑63 (MQ=255)                                   gTGCCGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAAc                              <  1:1014664/70‑1 (MQ=255)                                       cGTTCACACCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTa                         <  1:1093678/71‑1 (MQ=255)                                            acacCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCg                    <  1:6044/71‑1 (MQ=255)                                            acacCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCg                    <  1:754982/71‑1 (MQ=255)                                              acCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGt                  <  1:1792966/71‑1 (MQ=255)                                              acCGCGCCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGt                  <  1:160627/71‑1 (MQ=255)                                                 gcgcCTGGCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGGTg                >  1:1472515/1‑70 (MQ=255)                                                        gCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGa         >  1:2211610/1‑70 (MQ=255)                                                        gCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGAc        <  1:534149/71‑1 (MQ=255)                                                        gCCACGGCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGAc        <  1:1058500/71‑1 (MQ=255)                                                        gCCACGGCCCGAGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGAc        <  1:850925/71‑1 (MQ=255)                                                             ggCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTgg               <  1:428163/59‑1 (MQ=255)                                                             ggCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCAc   <  1:2266571/71‑1 (MQ=255)                                                              gCCCGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACt  >  1:828313/1‑71 (MQ=255)                                                               cccGGGCGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGt                  >  1:2675829/1‑54 (MQ=255)                                                                    |                                                                 CTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACT  >  minE/21023‑21154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑