| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,522,743 | T→C | I121V (ATC→GTC) | pdxB ← | erythronate‑4‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,522,743 | 0 | T | C | 98.6% | 256.7 / ‑4.2 | 72 | I121V (ATC→GTC) | pdxB | erythronate‑4‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (38/32); total (39/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAA > minE/1522681‑1522808 | tttAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCg > 1:939860/1‑71 (MQ=255) ttAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGCCGTCCAACGTTACCAACGCCCACGCCCCCCACCg > 1:1458141/1‑70 (MQ=255) tCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTAc < 1:1604280/69‑1 (MQ=255) cTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCg < 1:761837/71‑1 (MQ=255) tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTa > 1:100307/1‑64 (MQ=255) tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTa > 1:1451270/1‑64 (MQ=255) tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTa > 1:242287/1‑64 (MQ=255) aCGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTAc < 1:1167621/63‑1 (MQ=255) aCGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTa < 1:1937742/70‑1 (MQ=255) gCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAg > 1:1126927/1‑71 (MQ=255) tCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTa < 1:2330028/57‑1 (MQ=255) tCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTa < 1:1909766/57‑1 (MQ=255) tCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTa < 1:1426230/57‑1 (MQ=255) ccAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTa < 1:2399067/56‑1 (MQ=255) cAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaaa < 1:2843220/71‑1 (MQ=255) cAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaa > 1:1409847/1‑70 (MQ=255) aGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCGCCACCGTACGGTCGTACAGTGaaaa > 1:1054213/1‑71 (MQ=255) aGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaaaa > 1:383252/1‑71 (MQ=255) aGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaaaa > 1:1164572/1‑71 (MQ=255) aGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaaaa > 1:2472905/1‑71 (MQ=255) aGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaa < 1:976275/69‑1 (MQ=255) aGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaa < 1:2800828/69‑1 (MQ=255) gTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAAt > 1:3155659/1‑71 (MQ=255) gTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAAt > 1:2596798/1‑71 (MQ=255) gcgcCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCa < 1:654147/71‑1 (MQ=255) cgcCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCa < 1:53359/70‑1 (MQ=255) gcCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATc > 1:94798/1‑71 (MQ=255) gcCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATc > 1:2426968/1‑71 (MQ=255) cTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaa < 1:1777468/61‑1 (MQ=255) cTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaa < 1:2476398/61‑1 (MQ=255) gCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCa > 1:947209/1‑65 (MQ=255) gCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGt > 1:2580561/1‑71 (MQ=255) cAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGt < 1:1271560/70‑1 (MQ=255) aaTCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTc > 1:1490778/1‑71 (MQ=255) tCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATcgcg < 1:1857063/66‑1 (MQ=255) gACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCg < 1:1138826/68‑1 (MQ=255) gACCTCCAACCTTACCAACGCCCACGACCCCCACCGTACGGTCCTACAGTGAAAATCCATCGCGTTCGGc > 1:1993557/1‑70 (MQ=255) cGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGc > 1:2993358/1‑68 (MQ=255) cGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGc > 1:2697199/1‑68 (MQ=255) cGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGc > 1:2606337/1‑68 (MQ=255) tCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCa < 1:2597958/71‑1 (MQ=255) ccAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGt < 1:2492760/60‑1 (MQ=255) aaCGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCAt > 1:1225424/1‑69 (MQ=255) aaCGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCAt > 1:2003325/1‑69 (MQ=255) aCGTTACCAACGCCCACGACCCCCACCGTACGGTCGt < 1:65911/37‑1 (MQ=255) aCGTTACCAACGCCCACGACCCCCACCGTACGGTCGt < 1:1755425/37‑1 (MQ=255) aCGTTACCAACGCCCACGACCCCCACCGTACGGTCGt < 1:1843504/37‑1 (MQ=255) aCGTTACCAACGCCCACGACCCCCACCGTACGGTCGt < 1:2598604/37‑1 (MQ=255) aCGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag < 1:1985435/71‑1 (MQ=255) aCGTTACCAACGCCCACGACCCACACCGTACGGTCGt < 1:1761447/37‑1 (MQ=38) aCCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCg < 1:955086/55‑1 (MQ=255) aCCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAgg > 1:2600809/1‑70 (MQ=255) aCCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAgg > 1:3106140/1‑70 (MQ=255) aCCAACGCCCACGA‑CCCCACCGTACGGTCGTACAGTGAAACTCCATCGCGTTCGGCAAGCATCAGCAgg > 1:1313237/1‑69 (MQ=255) ccAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAgg > 1:684222/1‑69 (MQ=255) ccAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGgga < 1:1335999/71‑1 (MQ=255) aCGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGggag > 1:431871/1‑69 (MQ=255) aCGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGggag > 1:1467210/1‑69 (MQ=255) aCGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGggag > 1:2263257/1‑69 (MQ=255) aCGCCCACGACCCCCACCGTAAGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGggag > 1:1319162/1‑69 (MQ=255) cGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTc < 1:2018777/49‑1 (MQ=255) cGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGggag > 1:2990618/1‑68 (MQ=255) cccACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcagca < 1:2515043/61‑1 (MQ=255) cccACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcagca < 1:661642/61‑1 (MQ=255) cccACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcagca < 1:1779873/61‑1 (MQ=255) cACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGaaaa > 1:2238034/1‑71 (MQ=255) cACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGaaaa < 1:2065874/71‑1 (MQ=255) cACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGaaaa > 1:775135/1‑71 (MQ=255) cACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGaaaa > 1:1899877/1‑71 (MQ=255) cACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGaaaa > 1:986978/1‑71 (MQ=255) cGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag > 1:2300209/1‑55 (MQ=255) cGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag > 1:1106579/1‑55 (MQ=255) | TTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAA > minE/1522681‑1522808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |