Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,735,602	T→C	E157E (GAA→GAG)	era ←	membrane‑associated, 16S rRNA‑binding GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,735,602	0	T	C	100.0%	227.5 / NA	61	E157E (GAA→GAG)	era	membrane‑associated, 16S rRNA‑binding GTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/25);  total (36/25)

AGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCG  >  minE/1735535‑1735663                                                                    |                                                              aGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCg                                                            <  1:419118/71‑1 (MQ=255) aGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCg                                                            >  1:388639/1‑71 (MQ=255)  gTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg                                                           >  1:1600121/1‑71 (MQ=255)  gTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg                                                           <  1:1778950/71‑1 (MQ=255)    gatgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg                                                           >  1:2397564/1‑69 (MQ=255)     atgaGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCgg                                                           >  1:2405795/1‑68 (MQ=255)        aGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCagag                                                      <  1:1216473/70‑1 (MQ=255)        aGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCagag                                                      <  1:245883/70‑1 (MQ=255)        aGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCa                                                         >  1:761645/1‑67 (MQ=255)         gTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGAt                                                    <  1:210125/71‑1 (MQ=255)            gCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTgg                                                 >  1:2618941/1‑71 (MQ=255)            gCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTgg                                                 >  1:2041546/1‑71 (MQ=255)              ttCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCagag                                                      <  1:3077170/64‑1 (MQ=255)                cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGAt                                                    >  1:2601633/1‑64 (MQ=255)                cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCa                                               <  1:2624819/69‑1 (MQ=255)                cAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCa                                               <  1:2550575/69‑1 (MQ=255)                 aGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACg                                             <  1:2150054/70‑1 (MQ=255)                   gTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGata                                          >  1:685246/1‑71 (MQ=255)                   gTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGata                                          >  1:834400/1‑71 (MQ=255)                   gTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGata                                          >  1:2493306/1‑71 (MQ=255)                   gTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGat                                           >  1:428492/1‑70 (MQ=255)                     aGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGa                                            >  1:695101/1‑67 (MQ=255)                     aGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGa                                            >  1:2045815/1‑67 (MQ=255)                           ttACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAgg                                    >  1:2316884/1‑69 (MQ=255)                             aCGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAg                                 <  1:2159321/70‑1 (MQ=255)                               gCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTc                              <  1:2834769/71‑1 (MQ=255)                                cACGATTGCCGCAATAGTGTCAACATTCAGCTCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa                             <  1:2695606/71‑1 (MQ=255)                                cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa                             <  1:2058145/71‑1 (MQ=255)                                cACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa                             <  1:2340028/71‑1 (MQ=255)                                 aCGATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCa                             <  1:1652850/70‑1 (MQ=255)                                   gATTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCt                          >  1:2160482/1‑71 (MQ=255)                                    aTTGCCGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTg                         >  1:1040080/1‑71 (MQ=255)                                        ccGCAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACTATATCGAGGAAGTTCATCTgg                        <  1:1332419/68‑1 (MQ=255)                                           cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGAt                                                    >  1:386437/1‑37 (MQ=255)                                           cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc                   >  1:2102061/1‑70 (MQ=255)                                           cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGc                   >  1:723086/1‑70 (MQ=255)                                           cAATAGTGTCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATATGGCTTGc                   >  1:2586668/1‑70 (MQ=255)                                                   tCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACt           <  1:815340/70‑1 (MQ=255)                                                   tCAACATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACt           <  1:417022/70‑1 (MQ=255)                                                     aaCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc                  <  1:1323238/61‑1 (MQ=255)                                                     aaCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc                  <  1:2788959/61‑1 (MQ=255)                                                     aaCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc                  <  1:43284/61‑1 (MQ=255)                                                      aCATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGcc                  <  1:1964292/60‑1 (MQ=255)                                                       cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt                            >  1:637570/1‑49 (MQ=255)                                                       cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt                            >  1:56477/1‑49 (MQ=255)                                                       cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAgg      >  1:1629577/1‑71 (MQ=255)                                                       cATTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAgg      >  1:2905866/1‑71 (MQ=255)                                                        aTTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGt     >  1:1768159/1‑71 (MQ=255)                                                        aTTCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGt     <  1:2212577/71‑1 (MQ=255)                                                         ttCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATc                           <  1:2291468/48‑1 (MQ=255)                                                         ttCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAg       >  1:2929809/1‑68 (MQ=255)                                                          tCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTc                              <  1:2722879/44‑1 (MQ=255)                                                          tCAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGc   >  1:1863666/1‑71 (MQ=255)                                                           cAGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCg  >  1:1551773/1‑71 (MQ=255)                                                            aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt                            >  1:1103039/1‑44 (MQ=255)                                                            aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt                            >  1:579544/1‑44 (MQ=255)                                                            aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt                            >  1:3025873/1‑44 (MQ=255)                                                            aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt                            >  1:2667318/1‑44 (MQ=255)                                                            aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt                            >  1:174396/1‑44 (MQ=255)                                                            aGCCCGGTCTCGGCAGAGATTGGCACGATATCGAGGAAGTTCAt                            >  1:1830756/1‑44 (MQ=255)                                                               ccGGTCTCGGCAGACATTGGCACGATATCGAGGaa                                  >  1:468586/1‑35 (MQ=255)                                                                    |                                                              AGTGATGAGTCGCTTCAGGTAGATGCTTACGCACGATTGCCGCAATAGTGTCAACATTCAGCCCGGTTTCGGCAGAGATTGGCACGATATCGAGGAAGTTCATCTGGCTTGCCAGGAACTGCAGGTGCG  >  minE/1735535‑1735663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑