| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,738,791 | G→A | C340C (TGC→TGT) | lepA ← | GTP binding membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,738,791 | 0 | G | A | 96.9% | 97.8 / ‑3.7 | 32 | C340C (TGC→TGT) | lepA | GTP binding membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (17/14); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
ATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCA > minE/1738725‑1738855 | atccagatcGTATACACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGc > 1:1094150/1‑71 (MQ=255) ccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCg > 1:593193/1‑70 (MQ=255) cGTATTCACGTTCGAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGa < 1:2593877/66‑1 (MQ=255) cGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGa < 1:2827152/66‑1 (MQ=255) gTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCaa < 1:1514929/71‑1 (MQ=255) tATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGa < 1:715599/64‑1 (MQ=255) cACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGcc > 1:314723/1‑70 (MQ=255) aCGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCa < 1:880060/71‑1 (MQ=255) aCGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCa < 1:461505/71‑1 (MQ=255) cGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAg > 1:2344242/1‑54 (MQ=255) ccAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGaaa > 1:562398/1‑56 (MQ=255) ccAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGaaa > 1:2185612/1‑56 (MQ=255) ccAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGaaa > 1:2442109/1‑56 (MQ=255) cAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGcc > 1:1731096/1‑63 (MQ=255) tCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGa > 1:213462/1‑46 (MQ=255) tGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTc > 1:2613368/1‑71 (MQ=255) tGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTc > 1:2749997/1‑71 (MQ=255) gatgatCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCg < 1:2259992/71‑1 (MQ=255) atCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAAc < 1:1079040/40‑1 (MQ=255) atCTCCATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAAc < 1:1286340/40‑1 (MQ=255) cATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCAt < 1:805240/68‑1 (MQ=255) cATGTGCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAg > 1:584907/1‑70 (MQ=255) cATGTGCAGCAGGCCCAGGAAGCCGCAGCGTATGCCGAAGCCCAGCGC‑CTGCGAGACTTCCGGCTcg < 1:780849/67‑2 (MQ=255) tgtgCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCt > 1:1893171/1‑49 (MQ=255) tgtgCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAGaa > 1:220868/1‑70 (MQ=255) tgCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAgcgcgc < 1:1978238/46‑1 (MQ=255) tgCAGCAGGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAGAACAg > 1:690600/1‑71 (MQ=255) cagcagGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAGAACAGTg < 1:1968658/71‑1 (MQ=255) cagcagGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAGAACAGTg < 1:1765068/71‑1 (MQ=255) agcagGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAGAACAGt > 1:3001756/1‑69 (MQ=255) agcagGCCGAGGAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAGAACAGt > 1:2597830/1‑69 (MQ=255) gAAGCCACAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCa < 1:2108732/71‑1 (MQ=255) | ATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTG‑GAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCA > minE/1738725‑1738855 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |