Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,744,552 G→A V483I (GTC→ATC)  nadB → quinolinate synthase, L‑aspartate oxidase (B protein) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,744,5520GA100.0% 126.5 / NA 39V483I (GTC→ATC) nadBquinolinate synthase, L‑aspartate oxidase (B protein) subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/20);  total (19/20)

TGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGC  >  minE/1744484‑1744616
                                                                    |                                                                
tGGAACGCGCCCTGCGGCGGATAACCCTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc                                                                >  1:28915/1‑71 (MQ=255)
       cgcCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAaata                                                         <  1:3069642/71‑1 (MQ=255)
              cggcggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGc                                                   <  1:606062/70‑1 (MQ=255)
              cggcggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGc                                                   <  1:2582080/70‑1 (MQ=255)
              cggcggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGc                                                   <  1:2242435/70‑1 (MQ=255)
               ggcggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTg                                                 <  1:2383510/71‑1 (MQ=255)
                  ggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtct                                                               >  1:146664/1‑54 (MQ=255)
                  ggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtct                                                               >  1:2552118/1‑54 (MQ=255)
                  ggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg                                              <  1:2009507/71‑1 (MQ=255)
                      aaCCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTg                                           <  1:2016073/70‑1 (MQ=255)
                        ccATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCg                                         >  1:1066023/1‑70 (MQ=255)
                        ccATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCg                                         >  1:498598/1‑70 (MQ=255)
                         cATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCg                                         >  1:954476/1‑69 (MQ=255)
                         cATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGt                                        >  1:779464/1‑70 (MQ=255)
                         cATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTa                                       <  1:1445781/71‑1 (MQ=255)
                         cATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTa                                       <  1:1876162/71‑1 (MQ=255)
                          aTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTa                                       >  1:470436/1‑70 (MQ=255)
                             cTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATc                                    <  1:636048/70‑1 (MQ=255)
                                  acaaGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTa                               >  1:3009207/1‑70 (MQ=255)
                                          tAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGcc                      <  1:2236824/71‑1 (MQ=255)
                                          tAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGcc                      <  1:322486/71‑1 (MQ=255)
                                            gACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGa                    <  1:943988/71‑1 (MQ=255)
                                            gACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGa                    <  1:2839646/71‑1 (MQ=255)
                                               gAATATTACGCCCATTTCCGCATCTCAAATAATTTGCt                                                  <  1:2528110/38‑1 (MQ=255)
                                               gAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACGGGTTGCCGAGtt                 <  1:1885325/71‑1 (MQ=255)
                                               gAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGtt                 >  1:1855634/1‑71 (MQ=255)
                                                    ttACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTg            <  1:23753/71‑1 (MQ=255)
                                                     tACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg                                              >  1:2828693/1‑36 (MQ=255)
                                                     tACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg                                              >  1:1897605/1‑36 (MQ=255)
                                                     tACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg                                              >  1:1735590/1‑36 (MQ=255)
                                                     tACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg                                              >  1:1662558/1‑36 (MQ=255)
                                                     tACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg                                              >  1:1459572/1‑36 (MQ=255)
                                                         cccATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGc       >  1:172714/1‑71 (MQ=255)
                                                            aTTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTg                                  >  1:2517552/1‑41 (MQ=255)
                                                            aTTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGa               <  1:2513297/60‑1 (MQ=255)
                                                             tttCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt    <  1:3192139/70‑1 (MQ=255)
                                                             tttCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt    <  1:618706/70‑1 (MQ=255)
                                                               tCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGc  >  1:508526/1‑70 (MQ=255)
                                                               tCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGc  >  1:1903792/1‑70 (MQ=255)
                                                                    |                                                                
TGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGC  >  minE/1744484‑1744616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: