Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,751,256	G→A	intergenic (‑42/‑165)	yfiF ← / → trxC	predicted methyltransferase/thioredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,751,256	0	G	A	100.0%	74.4 / NA	23	intergenic (‑42/‑165)	yfiF/trxC	predicted methyltransferase/thioredoxin 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

ACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAA  >  minE/1751194‑1751320                                                               |                                                                 aCCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCcg                                                           <  1:2083048/70‑1 (MQ=255)     ttcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCgg                                                      >  1:1050791/1‑71 (MQ=255)        atttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGtt                                                    <  1:1796669/70‑1 (MQ=255)           tcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGc                                                 <  1:3169992/70‑1 (MQ=255)            catCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGcc                                                <  1:2917780/70‑1 (MQ=255)             atCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCaaa                                                                >  1:449958/1‑53 (MQ=255)             atCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCaaa                                                                >  1:2267548/1‑53 (MQ=255)                    aTGCTTTTCTCCACCAACGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTc                                        <  1:1135114/70‑1 (MQ=255)                        ttttCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTAc                                    >  1:2211833/1‑70 (MQ=255)                           tctcCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCa                                  >  1:751286/1‑69 (MQ=255)                            ctcCACCAGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCaaa                                <  1:1126439/70‑1 (MQ=255)                               caccaGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCt                            <  1:1108335/71‑1 (MQ=255)                                  caGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc                          >  1:879346/1‑70 (MQ=255)                                  caGCGCTGCGCGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc                          >  1:54655/1‑70 (MQ=255)                                         gcgcGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTc                                        >  1:1875798/1‑49 (MQ=255)                                         gcgcGAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTc                                        >  1:476166/1‑49 (MQ=255)                                            cgAAGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATAc               <  1:10473/71‑1 (MQ=255)                                               aGCGCGCAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTa            <  1:1516470/71‑1 (MQ=255)                                                     cAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGaa       >  1:2710219/1‑70 (MQ=255)                                                     cAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGaa       >  1:2867414/1‑70 (MQ=255)                                                     cAGATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGaa       >  1:1669720/1‑70 (MQ=255)                                                       gATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaa    <  1:2826029/71‑1 (MQ=255)                                                       gATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaa    <  1:223413/71‑1 (MQ=255)                                                       gATTACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaa    <  1:1694210/71‑1 (MQ=255)                                                          tACCCAAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaaaa  >  1:862242/1‑70 (MQ=255)                                                               |                                                                 ACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAA  >  minE/1751194‑1751320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑