Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,813,392	G→A	R595R (CGC→CGT)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,813,392	0	G	A	100.0%	269.7 / NA	82	R595R (CGC→CGT)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (60/22);  total (60/22)

TGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAG  >  minE/1813325‑1813457                                                                    |                                                                  tgtTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAacgc                                                                <  1:1773424/71‑1 (MQ=255) tgtTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAacgc                                                                <  1:732827/71‑1 (MQ=255) tgtTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGCGAGAAGTCGAAacg                                                                 <  1:1691078/70‑1 (MQ=255)  gtTCACCAGGTCTTCGACCGCACGAATCTCT‑‑TGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACgcag                                                              >  1:2084655/1‑70 (MQ=255)    tCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACgcag                                                              >  1:1941823/1‑70 (MQ=255)        cAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCt                                                         >  1:1785260/1‑71 (MQ=255)         aGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCtt                                                        >  1:322435/1‑71 (MQ=255)         aGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCtt                                                        >  1:1569018/1‑71 (MQ=255)          ggTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTg                                                       >  1:951447/1‑71 (MQ=255)          ggTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTg                                                       >  1:1657266/1‑71 (MQ=255)          ggTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTg                                                       <  1:942719/71‑1 (MQ=255)            tCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTc                                                     >  1:105623/1‑71 (MQ=255)            tCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTc                                                     >  1:1012260/1‑71 (MQ=255)            tCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTc                                                     >  1:3072073/1‑71 (MQ=255)            tCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTc                                                     >  1:2630904/1‑71 (MQ=255)             cTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCg                                                    >  1:37800/1‑71 (MQ=255)                cGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGt                                                   <  1:157760/69‑1 (MQ=255)                cGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGt                                                   <  1:184386/69‑1 (MQ=255)                 gACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTaa                                                <  1:1897717/71‑1 (MQ=255)                 cgacGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGt                                                   <  1:2764474/65‑1 (MQ=255)                   ccGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAAc                                               >  1:1704190/1‑70 (MQ=255)                   ccGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAAc                                               >  1:2166416/1‑70 (MQ=255)                   ccGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAAc                                               >  1:1458304/1‑70 (MQ=255)                    cGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTGACCa                                             <  1:86570/71‑1 (MQ=255)                    cGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCa                                             <  1:2109250/71‑1 (MQ=255)                    cGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCa                                             <  1:1906035/71‑1 (MQ=255)                      cACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGt                                           >  1:226143/1‑71 (MQ=255)                      cACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTAGTTAACCAGt                                           >  1:2961397/1‑71 (MQ=255)                        cGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTg                                          >  1:581794/1‑70 (MQ=255)                          aaTCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAAc                                       >  1:1493341/1‑71 (MQ=255)                           aTCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAAcc                                      >  1:671608/1‑71 (MQ=255)                           aTCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAAcc                                      >  1:3020678/1‑71 (MQ=255)                           aTCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAAcc                                      >  1:2831282/1‑71 (MQ=255)                           aTCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAAcc                                      >  1:478828/1‑71 (MQ=255)                            tctcTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCt                                     >  1:2622986/1‑71 (MQ=255)                             ctctTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGt                                                   >  1:2069745/1‑56 (MQ=255)                             ctctTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGt                                                   >  1:1343517/1‑56 (MQ=255)                               cttctGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTc                                  >  1:2591910/1‑71 (MQ=255)                               cttctGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTc                                  >  1:1741914/1‑71 (MQ=255)                                ttctGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCttt                                   <  1:247552/69‑1 (MQ=255)                                ttctGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCttt                                   <  1:317241/69‑1 (MQ=255)                                ttctGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCtt                                    <  1:1858233/68‑1 (MQ=255)                                ttctGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCtt                                    <  1:2239520/68‑1 (MQ=255)                                 tctGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTg                                >  1:2139271/1‑71 (MQ=255)                                     gTTTCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTc                                  <  1:1805589/65‑1 (MQ=255)                                       ttCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGtt                                                  >  1:3107886/1‑47 (MQ=255)                                       ttCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGtt                                                  >  1:1463046/1‑47 (MQ=255)                                       ttCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGtt                                                  >  1:1778037/1‑47 (MQ=255)                                       ttCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTg                                >  1:2164775/1‑65 (MQ=255)                                        tCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAg                                            >  1:1732760/1‑52 (MQ=255)                                        tCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACa                         >  1:584811/1‑71 (MQ=255)                                        tCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACa                         >  1:2997297/1‑71 (MQ=255)                                        tCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACa                         >  1:3074132/1‑71 (MQ=255)                                        tCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACa                         >  1:3170225/1‑71 (MQ=255)                                        tCATCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACa                         >  1:424990/1‑71 (MQ=255)                                          aTCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATg                       <  1:2219680/71‑1 (MQ=255)                                           tCGCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACAt                        >  1:2354342/1‑69 (MQ=255)                                            cGCTTCGTTGTGTGAGAAGTCAAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAg                     >  1:2227166/1‑71 (MQ=255)                                             gCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAg                     >  1:1790957/1‑70 (MQ=255)                                             gCTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAg                     >  1:412466/1‑70 (MQ=255)                                              cTTCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTa                   <  1:150292/71‑1 (MQ=255)                                                tCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTa                   >  1:1431183/1‑69 (MQ=255)                                                tCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTa                   >  1:2343478/1‑69 (MQ=255)                                                tCGTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTAc                  >  1:2189452/1‑70 (MQ=255)                                                  gTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAAc                                               >  1:2779902/1‑39 (MQ=255)                                                  gTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAAc                                               >  1:2536689/1‑39 (MQ=255)                                                  gTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAAc                                               >  1:2027699/1‑39 (MQ=255)                                                  gTTGTGTGAGAAGTCGAAACGCAGCACCTTGTCGTTAAc                                               >  1:386442/1‑39 (MQ=255)                                                  gTTGTGTGAGAAGTCGAAACGCAGCACCCTGTCGTTAAc                                               >  1:1491138/1‑39 (MQ=38)                                                     gtgtgAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGaa            >  1:2261959/1‑71 (MQ=255)                                                      tgtgAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGaa            <  1:1952345/70‑1 (MQ=255)                                                       gtgAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAAcc          >  1:2357392/1‑71 (MQ=255)                                                       gtgAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAAcc          >  1:3170834/1‑71 (MQ=255)                                                        tgAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAAcc          <  1:1359772/70‑1 (MQ=255)                                                        tgAGAAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAAcc          <  1:320252/70‑1 (MQ=255)                                                         gagaAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATa                           <  1:1828244/52‑1 (MQ=255)                                                         gagaAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATa                           <  1:163033/52‑1 (MQ=255)                                                         gagaAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTg        >  1:1033875/1‑71 (MQ=255)                                                          agaAGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTg        <  1:2061224/70‑1 (MQ=255)                                                            aaGTCGAAACGCAGCACCTTGTCGTTAACCAGTGACCCTTTCTGCGATACATGAGTACCCAGAACCTGgcg     >  1:2928346/1‑71 (MQ=255)                                                            aaGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCg                              >  1:2408863/1‑46 (MQ=255)                                                            aaGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGgcg     >  1:2931936/1‑71 (MQ=255)                                                            aaGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACATTTCTGCGATACATGAGTACCCAGAACCTGgcg     >  1:2165811/1‑71 (MQ=255)                                                             aGTCGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGgcgc    <  1:717879/71‑1 (MQ=255)                                                                cGAAACGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGgcgcag  >  1:608153/1‑70 (MQ=255)                                                                    |                                                                  TGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAG  >  minE/1813325‑1813457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑