Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	62,679	A→G	F260F (TTT→TTC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	62,679	0	A	G	100.0%	131.9 / NA	36	F260F (TTT→TTC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

TTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCT  >  minE/62614‑62746                                                                  |                                                                    ttCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGcc                                                                >  1:65594/1‑71 (MQ=255) ttCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGcc                                                                >  1:41517/1‑71 (MQ=255)       gcCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCt                                                           <  1:2846508/70‑1 (MQ=255)            gATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGcc                                                     <  1:1793935/71‑1 (MQ=255)               aCCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACt                                                  >  1:1153939/1‑71 (MQ=255)               aCCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACt                                                  >  1:1834253/1‑71 (MQ=255)                ccGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCCCGCCACTc                                                 <  1:1357856/71‑1 (MQ=255)                     gATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCt                                            <  1:1111253/71‑1 (MQ=255)                       tAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCg                                          >  1:59195/1‑71 (MQ=255)                       tAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCg                                          >  1:1609418/1‑71 (MQ=255)                        aaTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGc                                         >  1:1053668/1‑71 (MQ=255)                         aTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGc                                         <  1:889662/70‑1 (MQ=255)                          ttAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCta                                       <  1:2816133/71‑1 (MQ=255)                               cGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATc                                  <  1:997360/71‑1 (MQ=255)                               cGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATc                                  >  1:1126138/1‑71 (MQ=255)                                gACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATcg                                 <  1:982038/71‑1 (MQ=255)                                  cgtttAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACt                                                  <  1:2484375/50‑1 (MQ=255)                                  ccTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATcgcg                               <  1:1643712/71‑1 (MQ=255)                                   cTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATcgcg                               <  1:1595464/70‑1 (MQ=255)                                   cTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATcgcg                               <  1:2352225/70‑1 (MQ=255)                                   cTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATc                                  <  1:2170116/67‑1 (MQ=255)                                   cTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATc                                  <  1:787518/67‑1 (MQ=255)                                   cTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCgcgc                              >  1:1306753/1‑71 (MQ=255)                                   cTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCgcgc                              >  1:684942/1‑71 (MQ=255)                                   cTTTAGCCTGGGCAAAAAAGACGCCGGTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATcgcg                               <  1:1268294/70‑1 (MQ=255)                                     ttAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGcc                             >  1:2096347/1‑70 (MQ=255)                                     ttAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGcc                             >  1:2973873/1‑70 (MQ=255)                                               cAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCaa                          <  1:2245813/63‑1 (MQ=255)                                               cAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCaa                          <  1:299763/63‑1 (MQ=255)                                                aaaaaaGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTcc                                                <  1:2278341/40‑1 (MQ=255)                                                  aaaaGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCgg                >  1:1771803/1‑70 (MQ=255)                                                        cGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACgg         <  1:639145/71‑1 (MQ=255)                                                         gCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATcgc                                <  1:488168/47‑1 (MQ=255)                                                             tttttGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGaa              >  1:2729835/1‑61 (MQ=255)                                                               tttGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCt  >  1:1140329/1‑71 (MQ=255)                                                               tttGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCt  >  1:1060815/1‑71 (MQ=255)                                                                  |                                                                    TTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCT  >  minE/62614‑62746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑