Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,012,045	T→C	M63V (ATG→GTG)	yqhA ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,012,045	0	T	C	100.0%	371.7 / NA	99	M63V (ATG→GTG)	yqhA	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/50);  total (49/50)

GCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACG  >  minE/2011979‑2012112                                                                   |                                                                    gCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATcc                                                                 <  1:2436027/71‑1 (MQ=255) gCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATc                                                                  >  1:1876317/1‑70 (MQ=255)  cTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATcca                                                                >  1:2098304/1‑71 (MQ=255)  cTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATcc                                                                 >  1:1011960/1‑70 (MQ=255)  cTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATc                                                                  >  1:2514773/1‑69 (MQ=255)     tgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATccac                                                               <  1:1239618/69‑1 (MQ=255)     tgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATccac                                                               <  1:2699117/69‑1 (MQ=255)      gagaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAg                                                            >  1:503286/1‑71 (MQ=255)           gAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCgggt                                                       >  1:1277122/1‑68 (MQ=255)           gAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCggg                                                        >  1:926847/1‑68 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATccac                                                               >  1:2328828/1‑62 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATccac                                                               >  1:1689963/1‑62 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATccac                                                               >  1:3034623/1‑62 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg                                                      >  1:1408591/1‑71 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg                                                      >  1:1355999/1‑71 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg                                                      >  1:912460/1‑71 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg                                                      >  1:2534331/1‑71 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg                                                      >  1:2202031/1‑71 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg                                                      >  1:1144386/1‑71 (MQ=255)            aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAg                                                      >  1:2748735/1‑71 (MQ=255)              aTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGc                                                     <  1:1761389/70‑1 (MQ=255)               ttCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCaa                                                   >  1:1377713/1‑71 (MQ=255)                tctcATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCaa                                                   >  1:2000738/1‑70 (MQ=255)                  atttAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAacac                                                <  1:391347/68‑1 (MQ=255)                  atttAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAacac                                                <  1:1166338/68‑1 (MQ=255)                   cATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGc                                                     <  1:1513619/65‑1 (MQ=255)                   cATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGc                                                     <  1:189218/65‑1 (MQ=255)                    aTAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATc                                                                  <  1:2413315/51‑1 (MQ=255)                    aTAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAacac                                                >  1:1114064/1‑69 (MQ=255)                     tAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACa                                                       <  1:96800/61‑1 (MQ=255)                      aaCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAg                                             >  1:2172408/1‑70 (MQ=255)                       aCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAg                                                            >  1:903549/1‑54 (MQ=255)                       aCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGa                                           <  1:1105562/71‑1 (MQ=255)                            aaaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGa                                           <  1:2916232/66‑1 (MQ=255)                            aaaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGa                                           <  1:1966764/66‑1 (MQ=255)                            aaaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGa                                           <  1:1773664/66‑1 (MQ=255)                            aaaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCaaa                                      >  1:189753/1‑71 (MQ=255)                            aaaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCaaa                                      >  1:1946198/1‑71 (MQ=255)                            aaaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCaaa                                      >  1:168088/1‑71 (MQ=255)                            aaaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCaaa                                      >  1:692740/1‑71 (MQ=255)                             aaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGa                                                         >  1:844741/1‑51 (MQ=255)                             aaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt                                     <  1:3175173/71‑1 (MQ=255)                             aaaCATCACCATCACCAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt                                     <  1:2323905/71‑1 (MQ=255)                                 atcaccatcaccAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:1881993/71‑1 (MQ=255)                                 atcaccatcaccAGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:503631/71‑1 (MQ=255)                                  tcaccatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGAt                                >  1:1278882/1‑71 (MQ=255)                                  tcaccatcaccaGAAAACCGCCAACCAGCGTCACATccacca                                                             <  1:26592/42‑1 (MQ=37)                                     ccatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTcc                             <  1:2941484/71‑1 (MQ=255)                                     ccatcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTcc                             <  1:1675729/71‑1 (MQ=255)                                        tcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCg                                                          <  1:1038180/39‑1 (MQ=255)                                        tcaccaGCAAACCGCCAACCAGCGTCACATCCACCAGCg                                                          <  1:1078876/39‑1 (MQ=255)                                        tcaccaGCAAACCACCAACCAGCGTCACATCCACCAGCg                                                          <  1:1402876/39‑1 (MQ=37)                                          accaGCAAACCGCCAACCAGCGTCACATCCACCAGCGAc                                                        >  1:824892/1‑39 (MQ=255)                                          accaGCAAACCGCCAACCAGCGTCACATCCACCAGCGAc                                                        >  1:643598/1‑39 (MQ=255)                                          accaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCAt                        >  1:401670/1‑71 (MQ=255)                                           ccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt                                     <  1:1039345/57‑1 (MQ=255)                                           ccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt                                     <  1:2462264/57‑1 (MQ=255)                                           ccaGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt                                     <  1:2289210/57‑1 (MQ=255)                                            caGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGAt                                          <  1:322363/51‑1 (MQ=255)                                            caGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGAt                                          <  1:1296453/51‑1 (MQ=255)                                            caGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGAt                                          <  1:552565/51‑1 (MQ=255)                                            caGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt                                     <  1:1247510/56‑1 (MQ=255)                                            caGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc                       >  1:1059021/1‑70 (MQ=255)                                             aGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc                       >  1:386036/1‑69 (MQ=255)                                             aGCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc                       >  1:1573157/1‑69 (MQ=255)                                              gCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCa                                        >  1:2713766/1‑51 (MQ=255)                                              gCAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATc                       >  1:30818/1‑68 (MQ=255)                                               cAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgaga                   <  1:678422/71‑1 (MQ=255)                                               cAAACCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag                    >  1:1024663/1‑70 (MQ=255)                                                aaaCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaa                  <  1:178174/71‑1 (MQ=255)                                                aaaCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaa                  <  1:904966/71‑1 (MQ=255)                                                aaaCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaa                  <  1:228446/71‑1 (MQ=255)                                                 aaCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCa                         >  1:1474384/1‑63 (MQ=255)                                                 aaCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCa                         >  1:1616213/1‑63 (MQ=255)                                                 aaCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaag                 <  1:2421242/71‑1 (MQ=255)                                                  aCCGCCAACCAGCGTCACATCCACCAGCGACAGCAATACGAGGATCAAATCTGATTCCGCCATCGagaaga                <  1:1938390/71‑1 (MQ=255)                                                  aCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCgag                    >  1:2725545/1‑67 (MQ=255)                                                  aCCGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGagaaga                <  1:2455452/71‑1 (MQ=255)                                                    cGCCTACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt                                     <  1:3085069/48‑1 (MQ=38)                                                    cGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt                                     <  1:647025/48‑1 (MQ=255)                                                    cGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAAt                                     <  1:1981519/48‑1 (MQ=255)                                                    cGCCAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGata              >  1:723885/1‑71 (MQ=255)                                                      ccAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:2250846/50‑1 (MQ=255)                                                      ccAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:64865/50‑1 (MQ=255)                                                      ccAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:621822/50‑1 (MQ=255)                                                      ccAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:587218/50‑1 (MQ=255)                                                      ccAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:1644460/50‑1 (MQ=255)                                                      ccAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:2718418/50‑1 (MQ=255)                                                      ccAACCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:1163715/50‑1 (MQ=255)                                                        aaCCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg          >  1:2939735/1‑71 (MQ=255)                                                        aaCCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg          >  1:1957985/1‑71 (MQ=255)                                                        aaCCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCg          >  1:217662/1‑71 (MQ=255)                                                         aCCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCg                            >  1:1735225/1‑52 (MQ=255)                                                         aCCAGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCg                            >  1:247502/1‑52 (MQ=255)                                                            aGCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATACGGCAg      <  1:287269/71‑1 (MQ=255)                                                             gCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAg      >  1:56776/1‑70 (MQ=255)                                                             gCGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAg      >  1:2651818/1‑70 (MQ=255)                                                              cGTCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGa                                 <  1:1314398/42‑1 (MQ=255)                                                                tCACATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACg  >  1:1124648/1‑71 (MQ=255)                                                                   |                                                                    GCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACG  >  minE/2011979‑2012112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑