Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,207,332 A→G intergenic (‑22/+408) tldD ← / ← yhdP predicted peptidase/conserved membrane protein, predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,207,3320AG100.0% 121.3 / NA 34intergenic (‑22/+408)tldD/yhdPpredicted peptidase/conserved membrane protein, predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/9);  total (25/9)

CGTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATG  >  minE/2207268‑2207398
                                                                |                                                                  
cGTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCAACTCGTTAGTTGCTGCAg                                                              >  1:668436/1‑71 (MQ=255)
  ttCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTa                                                            <  1:465518/71‑1 (MQ=255)
         cTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTagag                                                         <  1:390756/67‑1 (MQ=255)
            gCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGAttt                                                    <  1:2936156/69‑1 (MQ=255)
                ttGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTa                                                            <  1:547062/57‑1 (MQ=255)
                ttGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTAc                                               <  1:1145056/70‑1 (MQ=255)
                     cACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTg                                          <  1:1607201/70‑1 (MQ=255)
                      aCTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTa                                                            >  1:2600937/1‑51 (MQ=255)
                      aCTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTa                                                            >  1:2001808/1‑51 (MQ=255)
                          aCCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGAtttttt                                                 >  1:1698837/1‑58 (MQ=255)
                             aGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAg                                                              >  1:2538292/1‑42 (MQ=255)
                             aGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAg                                                              >  1:1894497/1‑42 (MQ=255)
                             aGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAg                                                              >  1:1244683/1‑42 (MQ=255)
                             aGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAg                                                              >  1:1782030/1‑42 (MQ=255)
                                 tAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTAc                                               >  1:1006744/1‑53 (MQ=255)
                                 tAAGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTAc                                               >  1:1379811/1‑53 (MQ=255)
                                  aaGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGt                             >  1:1203362/1‑70 (MQ=255)
                                  aaGACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGt                             >  1:2504221/1‑70 (MQ=255)
                                    gACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGaa                          >  1:1866829/1‑71 (MQ=255)
                                    gACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGaa                          >  1:1098226/1‑71 (MQ=255)
                                    gACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGaa                          >  1:2486903/1‑71 (MQ=255)
                                    gACTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGaa                          >  1:2490651/1‑71 (MQ=255)
                                     aCTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAAc                         <  1:565998/71‑1 (MQ=255)
                                     aCTCATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAAc                         <  1:1017656/71‑1 (MQ=255)
                                        cATCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGcc                      >  1:1058735/1‑71 (MQ=255)
                                         aTCGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGccc                     >  1:1729044/1‑71 (MQ=255)
                                           cGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCt                    >  1:1098283/1‑70 (MQ=255)
                                           cGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTa                   >  1:1508665/1‑71 (MQ=255)
                                           cGTTTTTGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTa                   >  1:1435416/1‑71 (MQ=255)
                                               tttGCTACTCGTTAGTTGCTGCAGTAGAGGATTTTTTACgg                                             >  1:545339/1‑41 (MQ=255)
                                                     aCTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTAc         >  1:3079346/1‑71 (MQ=255)
                                                      cTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTa          <  1:74378/69‑1 (MQ=255)
                                                      cTCGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACg        >  1:2188330/1‑71 (MQ=255)
                                                        cGTTAGTTGCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGt      >  1:132840/1‑71 (MQ=255)
                                                            agttgCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATg  >  1:2008048/1‑71 (MQ=255)
                                                                |                                                                  
CGTTCGCCGCTAGCAATTGTTCACTTACCAGGTTAAGACTCATCGTTTTTGCTACTCGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATG  >  minE/2207268‑2207398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: