BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,281,121	A→G	F67S (TTT→TCT)	fabR ←	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,281,121	0	A	G	100.0%	196.1 / NA	54	F67S (TTT→TCT)	fabR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/23); total (31/23)

TAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGT  >  minE/2281054‑2281186

tAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaagaag                                                                <  1:2170684/71‑1 (MQ=255)

tAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaagaag                                                                <  1:2135579/71‑1 (MQ=255)

tAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaagaag                                                                <  1:1338481/71‑1 (MQ=255)

tAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATaagaag                                                                <  1:478568/71‑1 (MQ=255)

  gCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAagag                                                              <  1:335756/71‑1 (MQ=255)

  gCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAagag                                                              <  1:707904/71‑1 (MQ=255)

  gCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAagag                                                              <  1:2855404/71‑1 (MQ=255)

  gCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAagag                                                              <  1:615435/71‑1 (MQ=255)

     ttAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTg                                                           >  1:3170160/1‑71 (MQ=255)

     ttAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTg                                                           >  1:2738421/1‑71 (MQ=255)

     ttAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTg                                                           >  1:2843493/1‑71 (MQ=255)

      tAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTgg                                                          >  1:2509091/1‑71 (MQ=255)

       aaaCCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTgg                                                          >  1:713619/1‑70 (MQ=255)

        aaCCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTgg                                                          >  1:705647/1‑69 (MQ=255)

         aCCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAg                                                       <  1:2735735/71‑1 (MQ=255)

         aCCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAg                                                       <  1:1843081/71‑1 (MQ=255)

          ccGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTggggg                                                       >  1:1722721/1‑68 (MQ=255)

          ccGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAg                                                       >  1:2823636/1‑70 (MQ=255)

          ccGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAg                                                       >  1:1719236/1‑70 (MQ=255)

          ccGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAg                                                       >  1:2460555/1‑70 (MQ=255)

                  atcaACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGcc                                                <  1:2683800/69‑1 (MQ=255)

                  atcaACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCg                                              <  1:1510859/71‑1 (MQ=255)

                  atcaACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCg                                              <  1:2249058/71‑1 (MQ=255)

                  atcaACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCAATAAGAAGAGGTGGGAGCAATGcc                                                <  1:1178995/69‑1 (MQ=255)

                   tcaACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGc                                             >  1:2651171/1‑71 (MQ=255)

                     aaCCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCtt                                           >  1:2230130/1‑71 (MQ=255)

                      aCCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCtt                                           >  1:2425790/1‑70 (MQ=255)

                      aCCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCtt                                           >  1:2429465/1‑70 (MQ=255)

                      aCCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCtt                                           >  1:30466/1‑70 (MQ=255)

                        cATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCAc                                        <  1:841316/71‑1 (MQ=255)

                        cATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCAc                                        <  1:1865647/71‑1 (MQ=255)

                        cATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCAc                                        <  1:686119/71‑1 (MQ=255)

                          tGGTCAGACCCAGTTCGTCTACGTCGCGGTAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCa                                         >  1:1705739/1‑68 (MQ=255)

                          tGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTc                                          >  1:1459324/1‑67 (MQ=255)

                          tGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCa                                         >  1:2035843/1‑68 (MQ=255)

                          tGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCa                                         >  1:1984455/1‑68 (MQ=255)

                          tGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCa                                         >  1:1279353/1‑68 (MQ=255)

                             tCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAagag                                                              <  1:1095472/44‑1 (MQ=255)

                             tCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGcc                                   <  1:1722033/71‑1 (MQ=255)

                               aGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAgg                                                             >  1:1860817/1‑43 (MQ=255)

                               aGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCa                                  >  1:2095076/1‑70 (MQ=255)

                                  cccAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCa                                  >  1:730883/1‑67 (MQ=255)

                                  cccAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCa                                  >  1:2448717/1‑67 (MQ=255)

                                   ccAGTTCGTCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCa                             >  1:1611009/1‑71 (MQ=255)

                                           tCTACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt                     <  1:1871094/71‑1 (MQ=255)

                                             tACGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACt                     >  1:2556865/1‑69 (MQ=255)

                                               cGTCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAgg                 >  1:2691068/1‑71 (MQ=255)

                                                 tCGCGGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCt               <  1:1006401/71‑1 (MQ=255)

                                                    cgGAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGc            <  1:2005843/71‑1 (MQ=255)

                                                     ggAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACtt                               <  1:2674988/51‑1 (MQ=255)

                                                     ggAAATGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCg           <  1:2519138/71‑1 (MQ=255)

                                                       aaaTGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCg           >  1:1834127/1‑69 (MQ=255)

                                                       aaaTGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCg           >  1:2900773/1‑69 (MQ=255)

                                                          tGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCATGCAAACTCAGGCTGGCGAAGCt      <  1:264683/71‑1 (MQ=255)

                                                          tGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCt      <  1:2388954/71‑1 (MQ=255)

                                                          tGCCGATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCt      <  1:947686/71‑1 (MQ=255)

                                                              gATAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGt  >  1:2801966/1‑71 (MQ=255)

                                                               aTAAGAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGaa         >  1:864891/1‑63 (MQ=255)

TAGCATTAAACCGCTCTCATCAACCATGGTCAGACCCAGTTCGTCTACGTCGCGGAAATGCCGATAAAAAGAGGTGGGAGCAATGCCCGCTTCACGCGCCACTTCACGCAAACTCAGGCTGGCGAAGCTGCGT  >  minE/2281054‑2281186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`