Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,429,321	T→C	M88V (ATG→GTG)	wzzE ←	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,429,321	0	T	C	100.0%	206.5 / NA	56	M88V (ATG→GTG)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/26);  total (30/26)

GAGGCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACC  >  minE/2429254‑2429388                                                                    |                                                                    gAGGCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGtt                                                                  >  1:3014036/1‑71 (MQ=255)  aGGCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGttt                                                                 >  1:2108870/1‑71 (MQ=255)   ggCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGttt                                                                 >  1:1521997/1‑70 (MQ=255)   ggCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTg                                                                <  1:660013/71‑1 (MQ=255)      cAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAAc                                                             <  1:2111107/71‑1 (MQ=255)        gTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACg                                                            >  1:606911/1‑70 (MQ=255)           gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACa                                                        >  1:2748257/1‑71 (MQ=255)           gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACa                                                        >  1:1348344/1‑71 (MQ=255)           gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACa                                                        >  1:1554787/1‑71 (MQ=255)           gCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACa                                                        >  1:1658060/1‑71 (MQ=255)            cATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACAt                                                       <  1:220339/71‑1 (MQ=255)            cATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACAt                                                       <  1:1476365/71‑1 (MQ=255)                   aaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGtt                                                <  1:651124/71‑1 (MQ=255)                   aaCTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGt                                                 <  1:1964448/70‑1 (MQ=255)                        tttATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACg                                             <  1:1836118/69‑1 (MQ=255)                        tttATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACg                                             <  1:2357936/69‑1 (MQ=255)                         ttATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACCGACATCCAGGTTACGCa                                           >  1:914371/1‑70 (MQ=255)                          tataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaa                                          <  1:678267/70‑1 (MQ=255)                           ataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaaa                                         <  1:519466/70‑1 (MQ=255)                           ataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaaa                                         <  1:93666/70‑1 (MQ=255)                           ataGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaa                                          <  1:2052672/69‑1 (MQ=255)                            taGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCaaaaa                                       <  1:518391/71‑1 (MQ=255)                                  tCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCa                                                    >  1:2749378/1‑52 (MQ=255)                                  tCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAg                                                   >  1:1059805/1‑53 (MQ=255)                                  tCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAg                                                   >  1:1084770/1‑53 (MQ=255)                                  tCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAg                                                   >  1:2719754/1‑53 (MQ=255)                                  tCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAg                                                   >  1:658206/1‑53 (MQ=255)                                  tCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAg                                                   >  1:370708/1‑53 (MQ=255)                                  tCGTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTAGAACGGACATCCAg                                                   >  1:1311901/1‑53 (MQ=255)                                    gTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgct                               <  1:2524517/71‑1 (MQ=255)                                    gTCCATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgct                               <  1:601244/71‑1 (MQ=255)                                       cATGACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATtgctgctgc                             >  1:3063535/1‑70 (MQ=255)                                          gACCGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAg                          >  1:20596/1‑70 (MQ=255)                                             cGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGAc                                                         >  1:3020738/1‑36 (MQ=255)                                             cGATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCg                            <  1:791879/65‑1 (MQ=255)                                              gATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCg                            <  1:1537291/64‑1 (MQ=255)                                              gATGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAata                     <  1:965381/71‑1 (MQ=255)                                               aTGGTTGGTCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAatat                    <  1:167667/71‑1 (MQ=255)                                                  gttggtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa                                               <  1:3135221/41‑1 (MQ=255)                                                  gttggtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa                                               <  1:1809748/41‑1 (MQ=255)                                                  gttggtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa                                               <  1:2842975/41‑1 (MQ=255)                                                   ttgggCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCg                            <  1:244039/59‑1 (MQ=255)                                                    tggtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACg                                             >  1:920216/1‑41 (MQ=255)                                                    tggtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACg                                             >  1:1445921/1‑41 (MQ=255)                                                    tggtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATccccc               >  1:1947107/1‑71 (MQ=255)                                                    tggtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATccccc               >  1:1986807/1‑71 (MQ=255)                                                    tggtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATccc                 >  1:3101032/1‑69 (MQ=255)                                                    tggtAGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACg                                             >  1:2665893/1‑41 (MQ=255)                                                      gtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTa                                               <  1:1607025/37‑1 (MQ=255)                                                      gtCGGCAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATccccc               >  1:1556389/1‑69 (MQ=255)                                                           cAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCata        >  1:546201/1‑71 (MQ=255)                                                           cAGAAGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCata        >  1:1656256/1‑71 (MQ=255)                                                              aaGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAat                      <  1:1085304/54‑1 (MQ=255)                                                              aaGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATAtt      >  1:1078406/1‑70 (MQ=255)                                                              aaGCCACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATAtt      >  1:1864799/1‑70 (MQ=255)                                                                 ccACGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCAcc  <  1:1815001/71‑1 (MQ=255)                                                                    |                                                                    GAGGCCAGTTGCATAACAAACTCTTTATAGGCTTCGTCCATGACCGATGGTTGGTCGGCAGAAGCCATGTTTGAACGGACATCCAGGTTACGCAAAAATTGCTGCTGCGAGTAATATCCCCCCAGCATATTCACC  >  minE/2429254‑2429388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑