Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,463,313	G→A	V200V (GTG→GTA)	gidB →	methyltransferase, glucose‑inhibited cell‑division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,463,313	0	G	A	100.0%	39.9 / NA	13	V200V (GTG→GTA)	gidB	methyltransferase, glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/9);  total (4/9)

AGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCGGTTAGACTGT  >  minE/2463255‑2463379                                                           |                                                                   aGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGc                                                         <  1:1520838/70‑1 (MQ=255)      gAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATaa                                                   <  1:2373094/71‑1 (MQ=255)         tCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAaat                                                >  1:2629066/1‑71 (MQ=255)               gTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAaatttaatt                                          >  1:2986514/1‑71 (MQ=255)                 tAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAAtttt                                        >  1:972053/1‑71 (MQ=255)                           gTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCaaaaaa                              <  1:804384/71‑1 (MQ=255)                                 gCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATa                        <  1:3089995/71‑1 (MQ=255)                                    cTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATaaaa                     <  1:933656/71‑1 (MQ=255)                                           gCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCaaaaaaa                             <  1:860112/56‑1 (MQ=255)                                             gAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCg            >  1:561830/1‑71 (MQ=255)                                               aCGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCGGt          <  1:947369/71‑1 (MQ=255)                                                   cATCTGGTAGTGATTAAAGCAAATAAAAATTAATTTTTATCAAAAAAATCATAAAAAAt                  <  1:1718933/59‑1 (MQ=255)                                                    aTCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCGGTTAGa      <  1:2442779/70‑1 (MQ=255)                                                       tggtagTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCGGTTAGACTGt  >  1:2117587/1‑71 (MQ=255)                                                           |                                                                   AGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCGGTTAGACTGT  >  minE/2463255‑2463379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑