Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,512,108	C→T	C141Y (TGC→TAC)	yicE ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,512,108	0	C	T	100.0%	251.6 / NA	75	C141Y (TGC→TAC)	yicE	predicted transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/42);  total (33/42)

CGCCAGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGCAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAGCACCACCGGTT  >  minE/2512040‑2512173                                                                     |                                                                  cGCCAGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTaa                                                                 >  1:1848863/1‑71 (MQ=255)   ccAGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACt                                                               <  1:1554110/71‑1 (MQ=255)   ccAGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACt                                                               <  1:2869100/71‑1 (MQ=255)   ccAGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACt                                                               <  1:579874/71‑1 (MQ=255)     aGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTg                                                             >  1:1944036/1‑71 (MQ=255)     aGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTg                                                             >  1:2899498/1‑71 (MQ=255)     aGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTg                                                             >  1:2763795/1‑71 (MQ=255)     aGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTg                                                             >  1:2170492/1‑71 (MQ=255)      gAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGc                                                            >  1:1612546/1‑71 (MQ=255)       aaaCCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGcc                                                           <  1:1335170/71‑1 (MQ=255)       aaaCCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGcc                                                           <  1:1431575/71‑1 (MQ=255)       aaaCCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGcc                                                           <  1:53691/71‑1 (MQ=255)        aaCCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCa                                                          <  1:484966/71‑1 (MQ=255)            aGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCAt                                                      >  1:925492/1‑71 (MQ=255)             gcggcgTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCAt                                                      >  1:1772623/1‑70 (MQ=255)              cggcgTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCa                                                    <  1:2256220/71‑1 (MQ=255)               ggcgTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCa                                                    <  1:3144730/70‑1 (MQ=255)                  gTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGt                                                <  1:1968267/71‑1 (MQ=255)                        atGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGaa                                          >  1:335107/1‑71 (MQ=255)                        atGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGa                                           <  1:82697/70‑1 (MQ=255)                         tGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCa                                                    <  1:2349806/60‑1 (MQ=255)                         tGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCCCGGTGTAACTTGCCAGCATCAACGTGCCGAAc                                         <  1:1010410/71‑1 (MQ=255)                           cggcgCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACaa                                       <  1:1735014/71‑1 (MQ=255)                                   cAGATGCAGAACGCGGGAGATCACCCTCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCca                               >  1:628849/1‑71 (MQ=255)                                   cAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAgccgcc                                >  1:2863967/1‑70 (MQ=255)                                   cAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCca                               >  1:133991/1‑71 (MQ=255)                                   cAGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCca                               >  1:744349/1‑71 (MQ=255)                                    aGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcat                              <  1:1255983/71‑1 (MQ=255)                                    aGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcat                              <  1:2718780/71‑1 (MQ=255)                                    aGATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcat                              <  1:1156871/71‑1 (MQ=255)                                     gATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTg                                                             <  1:2003538/39‑1 (MQ=255)                                     gATGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTg                                                             <  1:2075009/39‑1 (MQ=255)                                      aTGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCaa                                                   >  1:2550173/1‑48 (MQ=255)                                      aTGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCaa                                                   >  1:2357309/1‑48 (MQ=255)                                      aTGCAGAACGCGGGAGATCACCATATCGGTGTAACTTGCCAGAATCaa                                                   >  1:2130884/1‑48 (MQ=255)                                       tGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcatcat                           <  1:2902799/71‑1 (MQ=255)                                       tGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcatcat                           <  1:686221/71‑1 (MQ=255)                                       tGCAGAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcatcat                           <  1:939938/71‑1 (MQ=255)                                           gAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACa                                        <  1:1099689/54‑1 (MQ=255)                                           gAACGCGGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACa                                        <  1:2204214/54‑1 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCaa                                                   >  1:1061137/1‑40 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGa                                           >  1:3145792/1‑48 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGa                                           >  1:1929373/1‑48 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGa                                           >  1:2032626/1‑48 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAgg                     >  1:1633182/1‑70 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAgg                     >  1:1629307/1‑70 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAgg                     >  1:609200/1‑70 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAgg                     >  1:2281648/1‑70 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAgg                     >  1:2426037/1‑70 (MQ=255)                                              cgcgGGAGATCACCATCTCGGTGTAACTTGCCACCATCAACGTGCCGa                                           >  1:1171767/1‑48 (MQ=255)                                               gcgGGAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGa                    >  1:736434/1‑70 (MQ=255)                                                 gggAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGa                    <  1:857735/68‑1 (MQ=255)                                                 gggAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACa                 <  1:845780/71‑1 (MQ=255)                                                 gggAGATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACa                 <  1:3193552/71‑1 (MQ=255)                                                 gggACATCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACa                 <  1:2906827/71‑1 (MQ=255)                                                    agaTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcatcat                           <  1:1473534/58‑1 (MQ=255)                                                    agaTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcatcat                           <  1:1046933/58‑1 (MQ=255)                                                    agaTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcatcat                           <  1:3053069/58‑1 (MQ=255)                                                    agaTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCcatcat                           <  1:1965322/58‑1 (MQ=255)                                                     gaTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAAc                                         <  1:3104273/43‑1 (MQ=255)                                                     gaTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAg             <  1:2456342/71‑1 (MQ=255)                                                      aTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGa                                           <  1:346887/40‑1 (MQ=255)                                                      aTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGa                                           <  1:1526711/40‑1 (MQ=255)                                                      aTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGa                                           <  1:601493/40‑1 (MQ=255)                                                      aTCACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGa                                           <  1:2860015/40‑1 (MQ=255)                                                        cACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAg             <  1:918059/68‑1 (MQ=255)                                                        cACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAg             <  1:1824626/68‑1 (MQ=255)                                                        cACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAg             <  1:1224381/68‑1 (MQ=255)                                                        cACCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAGcac          >  1:3189891/1‑71 (MQ=255)                                                         aCCATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAGcac          >  1:2344705/1‑70 (MQ=255)                                                          ccATCTCGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAGcacca        <  1:2062890/71‑1 (MQ=255)                                                          ccATCTCCGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAGcacca        >  1:2917284/1‑71 (MQ=255)                                                             tctcGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAGCACCACCg     >  1:2283022/1‑71 (MQ=255)                                                               tcGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAGCACCACCGGt   >  1:2323532/1‑71 (MQ=255)                                                                cGGTGTAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAGCACCACCGGtt  >  1:2931265/1‑71 (MQ=255)                                                                  gtgtAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAAc                  <  1:1160102/53‑1 (MQ=255)                                                                  gtgtAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAAc                  <  1:1241970/53‑1 (MQ=255)                                                                  gtgtAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAAc                  <  1:2384023/53‑1 (MQ=255)                                                                     |                                                                  CGCCAGAAACCAGCGGCGTAATAATGCGGCGCGCCAGATGCAGAACGCGGGAGATCACCATCTCGGTGCAACTTGCCAGCATCAACGTGCCGAACAAAGCCGCCATCATGGTAGGAACATCAGCACCACCGGTT  >  minE/2512040‑2512173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑