BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,523,621	C→T	G32S (GGT→AGT)	dinD ←	DNA‑damage‑inducible protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,523,621	0	C	T	100.0%	185.2 / NA	55	G32S (GGT→AGT)	dinD	DNA‑damage‑inducible protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/34); total (21/34)

CCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGT  >  minE/2523554‑2523679

ccACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCag                                                         <  1:1929312/71‑1 (MQ=255)

  aCTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCag                                                         >  1:1441128/1‑69 (MQ=255)

    tGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagtagt                                                     >  1:678748/1‑71 (MQ=255)

     gTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGtt                                                    >  1:2682067/1‑71 (MQ=255)

     gTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGtt                                                    >  1:1764854/1‑71 (MQ=255)

           acaGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTccc                                                >  1:1572058/1‑69 (MQ=255)

              ggCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGtt                                           <  1:2677418/71‑1 (MQ=255)

                cttcttTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGt                                         >  1:2297340/1‑71 (MQ=255)

                cttcttTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGt                                         >  1:545184/1‑71 (MQ=255)

                 ttcttTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAg                                             >  1:2672914/1‑66 (MQ=255)

                   cttTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCt                                       <  1:2121721/70‑1 (MQ=255)

                    tttGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCt                                       <  1:1451734/69‑1 (MQ=255)

                    tttGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTg                                     >  1:2989273/1‑71 (MQ=255)

                      tGGCGCGCGTTAATACATGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCtt                                      <  1:1535129/68‑1 (MQ=255)

                      tGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTg                                     <  1:1146447/69‑1 (MQ=255)

                      tGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTg                                     <  1:1894256/69‑1 (MQ=255)

                      tGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTg                                     <  1:1376065/69‑1 (MQ=255)

                      tGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTg                                     <  1:128141/69‑1 (MQ=255)

                       ggCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCt                                   <  1:3180002/70‑1 (MQ=255)

                          gcgcgTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagta                                                       <  1:2739417/47‑1 (MQ=255)

                          gcgcgTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagta                                                       <  1:3033373/47‑1 (MQ=255)

                          gcgcgTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagta                                                       <  1:2743061/47‑1 (MQ=255)

                          gcgcgTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagta                                                       <  1:811018/47‑1 (MQ=255)

                          gcgcgTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCagta                                                       <  1:1019875/47‑1 (MQ=255)

                            gcgTTAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCAt                             >  1:2376471/1‑71 (MQ=255)

                               ttAATACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGt                          >  1:550983/1‑71 (MQ=255)

                                 aaTACAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCt                                   >  1:1963890/1‑60 (MQ=255)

                                  aTACAGGTATAAAGTGACGATACTCTGAATAACTCa                                                          >  1:1994664/1‑36 (MQ=255)

                                     cAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTg                                          >  1:351660/1‑49 (MQ=255)

                                     cAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGc                     <  1:972498/70‑1 (MQ=255)

                                     cAGGTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCt                    <  1:468842/71‑1 (MQ=255)

                                       ggTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTcc                  >  1:3181424/1‑71 (MQ=255)

                                        gTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGtt                                           <  1:255479/45‑1 (MQ=255)

                                        gTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGtt                                           <  1:2652622/45‑1 (MQ=255)

                                        gTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGtt                                           <  1:2275838/45‑1 (MQ=255)

                                        gTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGtt                                           <  1:2248445/45‑1 (MQ=255)

                                        gTATAAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGtt                                           <  1:421492/45‑1 (MQ=255)

                                         tataAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGt                <  1:222570/71‑1 (MQ=255)

                                          ataAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGt                                         >  1:1461696/1‑45 (MQ=255)

                                          ataAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGt                                         >  1:2278275/1‑45 (MQ=255)

                                           taAAGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGtt               <  1:1181888/70‑1 (MQ=255)

                                              aGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCa           <  1:1490128/71‑1 (MQ=255)

                                              aGTGACGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCa           <  1:775759/71‑1 (MQ=255)

                                                  aCGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTa        <  1:3095527/70‑1 (MQ=255)

                                                  aCGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTa        <  1:140199/70‑1 (MQ=255)

                                                  aCGATACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTa        <  1:136391/70‑1 (MQ=255)

                                                      tACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAg   <  1:3002445/71‑1 (MQ=255)

                                                      tACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAg   <  1:2046675/71‑1 (MQ=255)

                                                      tACTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAg   <  1:2480215/71‑1 (MQ=255)

                                                       aCTCTGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAAt      >  1:1895399/1‑67 (MQ=255)

                                                       aCTCTGAATAACTCAGTAGTTTCACAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAAt      >  1:1750622/1‑67 (MQ=255)

                                                        ctctGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGt  <  1:409266/70‑1 (MQ=255)

                                                        ctctGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGt  <  1:1206579/70‑1 (MQ=255)

                                                           tGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGa                                 >  1:1590689/1‑36 (MQ=255)

                                                           tGAATAACTCAGTAGTTTCCCAAGTTGTCTTGCTGa                                 >  1:255990/1‑36 (MQ=255)

CCACTGTTCTCACAGGCTTCTTTGGCGCGCGTTAATACAGGTATAAAGTGACGATACTCTGAATAACCCAGTAGTTTCCCAAGTTGTCTTGCTGACCATTGTTCTGCTCCGTTTGCATTAATCAGT  >  minE/2523554‑2523679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`